Adaptation to herbivory by the Tammar wallaby includes bacterial and glycoside hydrolase profiles different from other herbivores

Pope, P. B., Denman, S. E., Jones, M., Tringe, S. G., Barry, K., Malfatti, S. A., McHardy, A. C., Cheng, J. F., Hugenholtz, P., McSweeney, C. S. and Morrison, M. (2010) Adaptation to herbivory by the Tammar wallaby includes bacterial and glycoside hydrolase profiles different from other herbivores. Proceedings of the National Academy of Sciences of the United States of America, 107 33: 14793-1-14798-6. doi:10.1073/pnas.1005297107


Author Pope, P. B.
Denman, S. E.
Jones, M.
Tringe, S. G.
Barry, K.
Malfatti, S. A.
McHardy, A. C.
Cheng, J. F.
Hugenholtz, P.
McSweeney, C. S.
Morrison, M.
Title Adaptation to herbivory by the Tammar wallaby includes bacterial and glycoside hydrolase profiles different from other herbivores
Journal name Proceedings of the National Academy of Sciences of the United States of America   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2010-08-01
Sub-type Article (original research)
DOI 10.1073/pnas.1005297107
Open Access Status Not Open Access
Volume 107
Issue 33
Start page 14793-1
End page 14798-6
Total pages 6
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Collection year 2011
Language eng
Abstract Metagenomic and bioinformatic approaches were used to characterize plant biomass conversion within the foregut microbiome of Australia's "model" marsupial, the Tammar wallaby (Macropus eugenii). Like the termite hindgut and bovine rumen, key enzymes and modular structures characteristic of the "free enzyme" and "cellulosome" paradigms of cellulose solubilization remain either poorly represented or elusive to capture by shotgun sequencing methods. Instead, multigene polysaccharide utilization loci-like systems coupled with genes encoding β-1,4-endoglucanases and β-1,4-endoxylanases - which have not been previously encountered in metagenomic datasets - were identified, as were a diverse set of glycoside hydrolases targeting noncellulosic polysaccharides. Furthermore, both rrs gene and other phylogenetic analyses confirmed that unique clades of the Lachnospiraceae, Bacteroidales, and Gammaproteobacteria are predominant in the Tammar foregut microbiome. Nucleotide composition-based sequence binning facilitated the assemblage of more than two megabase pairs of genomic sequence for one of the novel Lachnospiraceae clades (WG-2). These analyses show that WG-2 possesses numerous glycoside hydrolases targeting noncellulosic polysaccharides. These collective data demonstrate that Australian macropods not only harbor unique bacterial lineages underpinning plant biomass conversion, but their repertoire of glycoside hydrolases is distinct from those of the microbiomes of higher termites and the bovine rumen.
Keyword Cellulases
Marsupials
Metagenomics
Plant biomass conversion
Polysaccharide utilization loci
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 24 Mar 2011, 11:00:56 EST by Susan Allen on behalf of Institute for Molecular Bioscience