Alterations in gene expression in MEN1-associated insulinoma development

Serewko-Auret, MM, Mould, AW, Loffler, KA, Duncan, R, Kay, GF and Hayward, NK (2010) Alterations in gene expression in MEN1-associated insulinoma development. Pancreas, 39 8: 1140-1146. doi:10.1097/MPA.0b013e3181dc67fc

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Author Serewko-Auret, MM
Mould, AW
Loffler, KA
Duncan, R
Kay, GF
Hayward, NK
Title Alterations in gene expression in MEN1-associated insulinoma development
Journal name Pancreas   Check publisher's open access policy
ISSN 0885-3177
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1097/MPA.0b013e3181dc67fc
Volume 39
Issue 8
Start page 1140
End page 1146
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Lippincott Williams & Wilkins
Collection year 2011
Language eng
Formatted abstract
To identify gene expression alterations associated with insulinoma formation and progression in 2 mouse models of multiple endocrine neoplasia type 1.


Mice were killed at 12 or 16 months, and pancreatic islets were isolated by enzymatic and physical disruption. Islets were separated by size representing control, normal, hyperplastic, and adenomous islets. RNA was isolated from these islets and profiled on Sentrix Mouse-6 Expression version 1 BeadChips. Array data were analyzed in GeneSpring.

One hundred and one genes that were significantly (P ≤ 0.05) altered in hyperplastic islets and insulinomas compared with normal islets were identified. Of these, 64 gene elements showed reduced messenger RNA levels and 37 gene elements had increased gene expression compared with control islets. Altered expression of 3 genes, namely, Gata6, Tspan8, and s100a8, was confirmed by quantitative reverse transcription-polymerase chain reaction, and aberrant levels of Tspan8 and Lmo2 protein measured by Western blot correlated with the changes in messenger RNA levels.

These results suggest that alterations in gene expression of Gata6, Tspan8, S100a8, and Lmo2 may act via novel pathways that play functionally important roles in Men1-associated tumor progression. © 2010 by Lippincott Williams & Wilkins.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 23 Mar 2011, 13:29:20 EST by Lisa Hennell on behalf of Medicine - Royal Brisbane and Women's Hospital