Effect of nitrate and nitrite on the selection of microorganisms in the denitrifying anaerobic methane oxidation process

Hu, Shihu, Zeng, Raymond, Keller, Jurg, Lant, Paul A. and Yuan, Zhiguo (2011) Effect of nitrate and nitrite on the selection of microorganisms in the denitrifying anaerobic methane oxidation process. Environmental Microbiology Reports, 3 3: 315-319. doi:10.1111/j.1758-2229.2010.00227.x

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Author Hu, Shihu
Zeng, Raymond
Keller, Jurg
Lant, Paul A.
Yuan, Zhiguo
Title Effect of nitrate and nitrite on the selection of microorganisms in the denitrifying anaerobic methane oxidation process
Journal name Environmental Microbiology Reports   Check publisher's open access policy
ISSN 1758-2229
Publication date 2011-06
Year available 2010
Sub-type Article (original research)
DOI 10.1111/j.1758-2229.2010.00227.x
Volume 3
Issue 3
Start page 315
End page 319
Total pages 5
Editor Kenneth N. Timmis
David A. Stahl
Edward F. DeLong
Michael Wagner
Mike Jetten
Michael Galperin
Juan L. Ramos
Place of publication Oxford, U.K
Publisher Wiley-Blackwell Publishing
Collection year 2011
Language eng
Formatted abstract
Two cultures were inoculated with sludges taken from a parent culture containing archaea distantly related to anaerobic methanotrophic archaea (ANME) and bacteria related to Candidatus Methylomirabilis oxyfera, both of which have previously been found in cultures performing denitrifying anaerobic methane oxidation process. The cultures were fed with nitrate and nitrite, respectively, along with methane. The nitrate-fed culture, Culture B, showed a stable microbial community composition and denitrifying anaerobic methane oxidation activity. In contrast, the nitrite-fed culture, Culture A, achieved a substantial increase in the nitrite consumption rate, from 1.1 to 7.3 mmol NO2--N (g VSS)−1 day−1 in 90 days. Concurrent with this activity increase, the archaeal population in Culture A decreased with time, and became undetectable after 100 days, while bacteria related to M. oxyfera increasingly dominated the culture. This observation suggests that the bacteria related to M. oxyfera are more competitive for nitrite reduction compared with the archaea related to ANME. This study showed that nitrate or nitrite feeding has a significant impact on the ecology and activities of microorganisms in the denitrifying anaerobic methane oxidation process. This study also revealed that nitrite overloading may have a toxic effect on the bacteria related to M. oxyfera.
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 16 DEC 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
Official 2011 Collection
Advanced Water Management Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 23 Mar 2011, 10:20:39 EST by Hong Lee on behalf of Advanced Water Management Centre