Effect of astragalus membranaceus and angelica sinensis Combined with enalapril in rats with obstructive uropathy

Wojcikowski, Ken, Wohlmuth, Hans, Johnson, David W. and Gobe, Glenda (2010) Effect of astragalus membranaceus and angelica sinensis Combined with enalapril in rats with obstructive uropathy. Phytotherapy Research, 24 6: 875-884. doi:10.1002/ptr.3038


Author Wojcikowski, Ken
Wohlmuth, Hans
Johnson, David W.
Gobe, Glenda
Title Effect of astragalus membranaceus and angelica sinensis Combined with enalapril in rats with obstructive uropathy
Journal name Phytotherapy Research   Check publisher's open access policy
ISSN 0951-418X
Publication date 2010-06
Year available 2009
Sub-type Article (original research)
DOI 10.1002/ptr.3038
Volume 24
Issue 6
Start page 875
End page 884
Total pages 10
Place of publication United Kingdom
Publisher John Wiley & Sons Ltd.
Collection year 2011
Language eng
Abstract ACE inhibitors (ACEi) reduce renal tubulointerstitial fibrosis but are not completely effective. Combined extract of Astragalus membranaceus and Angelica sinensis (A&A) is a traditional antifibrotic agent in China. The present investigation aimed to determine whether an ACEi (Enalapril) and A&A together have a better antifibrotic effect in unilateral ureteral obstruction (UUO) than monotherapy with either agent. Male Sprague-Dawley rats (N = 4 per group) had either sham operation or UUO alone, with A&A (combined aqueous and ethanol extract equivalent to 2.1 g dried herbs), with Enalapril (in drinking water at 200 mg/mL) or with both treatments. Kidney and liver were collected for protein extraction or fixed for histologic stains, immunohistochemistry (IHC), microscopy. Enalapril or A&A individually were antifibrotic. Transforming growth factor-β1, fibroblast activation, collagen deposition, macrophage accumulation and tubular cell apoptosis were all decreased. The combination of the two drugs was significantly more effective than Enalapril alone in reducing tumor necrosis factor-α, collagen accumulation, activation of fibroblasts, and tubular cell apoptosis. In conclusion, Enalapril with A&A significantly decreased tubulointerstitial fibrosis to a greater extent than treatment with Enalapril alone. Further studies focusing on the isolation of the active constituents of A&A and the clinical application of the combination of ACEi plus A&A are warranted to determine the value of this treatment in humans. Copyright © 2009 John Wiley & Sons, Ltd.
Keyword A&A
Astragalus
Chronic kidney disease
Renal fibrosis
Transcribing growth factor beta-1
UUO
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
 
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Created: Tue, 22 Mar 2011, 13:31:53 EST by Debbie Banks on behalf of School of Medicine