The role of the vacB gene in the pathogenesis of Brucella abortus

Miyoshi, Anderson, Rosinha, Gracia M.S., Camargo, Ilana L.B.C., Trant, Cyntia M.C., Cardoso, Fernanda C., Azevedo, Vasco and Oliveira, Sergio C. (2007) The role of the vacB gene in the pathogenesis of Brucella abortus. Microbes and Infection, 9 3: 375-391. doi:10.1016/j.micinf.2006.12.004

Author Miyoshi, Anderson
Rosinha, Gracia M.S.
Camargo, Ilana L.B.C.
Trant, Cyntia M.C.
Cardoso, Fernanda C.
Azevedo, Vasco
Oliveira, Sergio C.
Title The role of the vacB gene in the pathogenesis of Brucella abortus
Journal name Microbes and Infection   Check publisher's open access policy
ISSN 1286-4579
Publication date 2007-03
Sub-type Article (original research)
DOI 10.1016/j.micinf.2006.12.004
Volume 9
Issue 3
Start page 375
End page 391
Total pages 17
Place of publication France
Publisher Elsevier Masson
Language eng
Abstract Brucella species are important zoonotic pathogens affecting a wide variety of mammals. Therefore, the identification of new Brucella virulence factors is of great interest in understanding bacterial pathogenesis and immune evasion. In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri vacB gene that encodes an exoribonuclease RNase R involved in bacterial virulence. Further, we have inactivated Brucella vacB by gene replacement strategy generating a deletion mutant strain. In order to test the role of Brucella vacB in pathogenesis, BALB/c and interferon regulatory factor-1 (IRF-1) knockout (KO) mice received Brucella vacB mutant, the virulent parental strain 2308 or the vaccine strain RB51 and the bacterial CFU numbers in spleens and mous survival were monitored. Our results demonstrated that the B. abortus ΔvacB mutant and the wild type strain 2308 showed similar CFU numbers in BALB/c mice. Additionally, IRF-1 KO mice that received either the vacB mutant or S2308 strain died in 12-14 days postinfection; in contrast, all animals that received the RB51 vaccine strain survived for 30 days postinoculation. In summary, this study reports that the vacB gene in B. abortus has no impact on bacterial pathogenesis. © 2007 Elsevier Masson SAS. All rights reserved.
Keyword Bacterial genes
Brucella abortus
vacB gene
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 22 Mar 2011, 12:24:52 EST by Susan Allen on behalf of Institute for Molecular Bioscience