Immunization with SmIg, a novel tegument protein from Schistosoma mansoni, fails to induce protection in mice but reduces liver pathology

Pinho, J. M. R., Cardoso, F. C., Lopes, D. O., Pinheiro, C. S., Caliari, M. V., Oliveira, F. M. S., Leite, L. C. and Oliveira, S. C. (2010) Immunization with SmIg, a novel tegument protein from Schistosoma mansoni, fails to induce protection in mice but reduces liver pathology. Parasitology, 137 07: 1079-1088. doi:10.1017/S0031182009991387


Author Pinho, J. M. R.
Cardoso, F. C.
Lopes, D. O.
Pinheiro, C. S.
Caliari, M. V.
Oliveira, F. M. S.
Leite, L. C.
Oliveira, S. C.
Title Immunization with SmIg, a novel tegument protein from Schistosoma mansoni, fails to induce protection in mice but reduces liver pathology
Formatted title
Immunization with SmIg, a novel tegument protein from Schistosoma mansoni, fails to induce protection in mice but reduces liver pathology
Journal name Parasitology   Check publisher's open access policy
ISSN 0031-1820
1469-8161
Publication date 2010-06-01
Sub-type Article (original research)
DOI 10.1017/S0031182009991387
Volume 137
Issue 07
Start page 1079
End page 1088
Total pages 10
Place of publication Cambridge, U.K.
Publisher Cambridge University Press
Collection year 2011
Language eng
Formatted abstract
Proteins associated with the schistosome tegument are of great importance for the development of new intervention strategies since they may be exposed on the surface of the parasite. Herein, we have isolated a cDNA clone encoding for the Schistosoma mansoni SmIg and its recombinant protein was tested as a potential vaccine candidate. Initially, its amino acid sequence was analysed by bioinformatics and shown to possess an N-terminal signal peptide, a C-terminal transmembrane helix, 4 glycosylation sites, an immunoglobulin conserved domain and 73% similarity with a hypothetical S. japonicum protein of unknown function. SmIg was produced by E. coli as a recombinant protein (rSmIg) and its protective effectiveness was evaluated against S. mansoni infection with 100 cercariae in a murine model. Mice immunized with rSmIg induced an immune response characterized by dominant IgG1 isotype and significant levels of IFN-γ, TNF-α, IL-10 and IL-4. Although immunogenic, the recombinant vaccine failed to induce worm burden reduction when compared to the infected control group. However, rSmIg-immunized mice had significant reductions of liver granuloma volume and fibrosis content by 31·8% and 49%, respectively. In conclusion, SmIg is a new tegument protein from S. mansoni that plays an important role in reducing pathology induced by parasite infection.
Keyword SmIg
Tegument protein
Vaccine
Schistosoma mansoni.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Published online: 16 October 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 22 Mar 2011, 21:57:43 EST by Susan Allen on behalf of Institute for Molecular Bioscience