Role of Nrf2 in retinal vascular development and the vaso-obliterative phase of oxygen-induced retinopathy

Uno, Koichi, Prow, Tarl, Bhutto, Imran A., Yerrapureddy, Adi, McLeod, D. Scott, Yamamoto, Masayuki, Reddy, Sekhar P. and Lutty, Gerard A. (2010) Role of Nrf2 in retinal vascular development and the vaso-obliterative phase of oxygen-induced retinopathy. Experimental Eye Research, 90 4: 493-500. doi:10.1016/j.exer.2009.12.012

Author Uno, Koichi
Prow, Tarl
Bhutto, Imran A.
Yerrapureddy, Adi
McLeod, D. Scott
Yamamoto, Masayuki
Reddy, Sekhar P.
Lutty, Gerard A.
Title Role of Nrf2 in retinal vascular development and the vaso-obliterative phase of oxygen-induced retinopathy
Journal name Experimental Eye Research   Check publisher's open access policy
ISSN 0014-4835
Publication date 2010-04
Sub-type Article (original research)
DOI 10.1016/j.exer.2009.12.012
Volume 90
Issue 4
Start page 493
End page 500
Total pages 8
Editor Joe Hollyfield
Place of publication Camden, London, U.K
Publisher Academic Press
Collection year 2011
Language eng
Formatted abstract
In the initial stage of retinopathy of prematurity (ROP), hyperoxia causes retinal blood vessel obliteration. This is thought to occur in part through oxidative stress-induced apoptosis of endothelial cells. This study was designed to determine what role NF-E2-related factor 2 (Nrf2) plays in this process. Nrf2 is a transcription factor of the anti-oxidant response element that, if induced, may protect the retina from hyperoxia-induced oxidative stress. Nrf2 knockout mice (Nrf2−/−), Nrf2 wild type control mice (Nrf2+/+), and C57BL/6 mice were exposed to hyperoxia (75% O2) or normoxia from P7 through P12. Mice were sacrificed on P9 and P12 and the retinas were stained with GSA lectin-Cy3 to visualize retinal blood vessels. Hyperoxia exposed retinas were flat mounted and photographed, then the size of the avascular areas was determined. Additionally, retinas were cryopreserved after lectin staining and area analysis and then sectioned. Secondary or deep capillaries were then hand-counted in sections. In hyperoxia-treated mice, the avascular areas in Nrf2−/− P9 mice were significantly larger than those in Nrf2+/+ P9 mice (P = 0.01). However, there was no significant difference between Nrf2−/− and Nrf2+/+ mice at P12. Avascular areas at P12 were significantly smaller than that at P9 in Nrf2−/−, Nrf2+/+, and C57BL/6 mice (P = 0.0011, P = 0.009, and P = 0.001 respectively). The numbers of deep or secondary capillaries in air-reared Nrf2−/− mice were significantly decreased, when compared to Nrf2+/+ mice at P9 (P = 0.0082). On the other hand, there was no significant difference in deep capillary formation between air-reared Nrf2−/− and Nrf2+/+ mice at P12. Akt signaling activates Nrf2 and Akt was localized to retinal blood vessels in all animals and was increased in Nrf2+/+ and Nrf2−/− mice exposed to hyperoxia as compared to normoxia mice. Interestingly, during normal development this protection by Nrf2 occurs in a specific window of time that is also shared by angiogenesis. Hyperoxia treatment revealed a similar window of time where Nrf2 regulated anti-oxidant production was beneficial and contributed to the endothelial survival.
© 2009 Published by Elsevier Ltd.
Keyword Blood vessels
Retinopathy of prematurity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Medicine Publications
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Created: Tue, 22 Mar 2011, 09:35:47 EST by Dr Tarl Prow on behalf of !NON-HERDC