Use of a meal challenge test to estimate peak postprandial triglyceride concentrations in dogs

Elliott, Kathryn F., Rand, Jacquie S., Fleeman, Linda M., Morton, John M. and Markwell, Peter J. (2011) Use of a meal challenge test to estimate peak postprandial triglyceride concentrations in dogs. American Journal of Veterinary Research, 72 2: 161-168. doi:10.2460/ajvr.72.2.161

Author Elliott, Kathryn F.
Rand, Jacquie S.
Fleeman, Linda M.
Morton, John M.
Markwell, Peter J.
Title Use of a meal challenge test to estimate peak postprandial triglyceride concentrations in dogs
Journal name American Journal of Veterinary Research   Check publisher's open access policy
ISSN 0002-9645
Publication date 2011-02
Sub-type Article (original research)
DOI 10.2460/ajvr.72.2.161
Volume 72
Issue 2
Start page 161
End page 168
Total pages 8
Place of publication Schaumberg IL, United States
Publisher American Veterinary Medical Association
Collection year 2012
Language eng
Formatted abstract
Objective—To develop a standardized meal challenge test by assessing associations between food-withheld preprandial (ie, fasting) and postprandial triglyceride concentrations, determining the most appropriate sampling time to detect the peak concentration (highest postprandial concentration), and estimating reference intervals for fasting and postprandial concentrations in healthy dogs.

Animals—12 lean healthy mixed-breed dogs.

Procedures—Dogs were fed a dry commercially available diet (fat, 31% metabolizable
energy) for 3 weeks. After food was withheld for 23 to 24 hours, plasma triglyceride
concentrations were measured 1 and 0.083 hours before and 1, 2, 3, 4, 5, 6, 9, and 12 hours after feeding of a standardized challenge meal (median amount eaten, 63 kcal/kg [127 kcal/ kg0.75]). Correlation and agreement between concentrations at peak and other time points were assessed by use of correlation coefficients and Bland-Altman limits of agreement. Reference intervals were calculated by use of a robust method.

Results—Fasting and peak triglyceride concentrations were not closely associated. The highest concentration among samples obtained 2, 5, and 6 hours after meal consumption had closest agreement with peak concentration. In 5 of 12 dogs, concentrations 12 hours after eating were still significantly above baseline concentration (mean of each dog’s fasting concentrations).

Conclusions and Clinical Relevance—
Fasting triglyceride concentration could not be used to accurately predict peak concentration. When estimating peak concentration, multiple samples should be collected 2, 5, and 6 hours after consumption of a standardized meal. Food may need to be withheld for > 12 hours when assessing fasting concentrations in healthy dogs. (Am J Vet Res 2011;72:161–168)
Keyword Healthy miniature-schnauzers
Nonfasting triglycerides
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 20 Mar 2011, 00:12:40 EST