Population structure of Sclerotinia sclerotiorum on sunflower in Australia

Ekins, M. G., Hayden, H. L., Aitken, E. A. B. and Goulter, K. C. (2011) Population structure of Sclerotinia sclerotiorum on sunflower in Australia. Australasian Plant Pathology, 40 2: 99-108. doi:10.1007/s13313-010-0018-6


Author Ekins, M. G.
Hayden, H. L.
Aitken, E. A. B.
Goulter, K. C.
Title Population structure of Sclerotinia sclerotiorum on sunflower in Australia
Formatted title
Population structure of Sclerotinia sclerotiorum on sunflower in Australia
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
1448-6032
Publication date 2011-03
Year available 2010
Sub-type Article (original research)
DOI 10.1007/s13313-010-0018-6
Volume 40
Issue 2
Start page 99
End page 108
Total pages 10
Editor Philip O'Brien
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2011
Language eng
Formatted abstract
Sunflower (Helianthus annuus) is host to infections by Sclerotinia sclerotiorum originating from either homothallic sexually-derived ascospores (stem and head rots) or asexually-derived sclerotia (root rot). While sunflower can be infected by either ascospores or sclerotia this study found no association between the genotypes found in lesions and the type of infection (stem, head or root rot). Multicopy Restriction Fragment Length Polymorphisms (RFLPs) showed individual sclerotia comprised of only one genotype, and that all eight ascospores within an ascus also had only one genotype. Mycelial Compatibility Groups (MCGs), Random Amplified Polymorphic DNAs (RAPDs), single and multicopy RFLP analyses all showed the majority of sunflower plants were infected by only one genotype. A sample of 250 isolates collected hierarchically from sunflowers in Queensland and New South Wales were shown to belong to one large genetic population of S. sclerotiorum. Temporal studies revealed genetic uniformity was maintained across years, further confirming one genetic population. A range of molecular markers were used to genotype 120 isolates, resulting in differing levels of resolution of a genotype. Between 13 and 24 genotypes were identified with similarities and differences in the assemblages of isolates within each genotype depending on the marker used.
© Australasian Plant Pathology Society Inc. 2010
Keyword Genetic diversity
Genotype
MCG
RAPD
RFLP
Population genetics
Clonal
Homothallic
Asexual
Sexual
Reproduction
Head rot
Basal stem rot
Ascospores
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Authors preprint tile: "Population structure of Sclerotinia sclerotiorum on sunflowers in Australia". Published online: 8 December 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Created: Sat, 19 Mar 2011, 00:49:22 EST by Dr Elizabeth Aitken on behalf of School of Biological Sciences