Alcian blue/alizarin red staining of cartilage and bone in mouse

Ovchinnikov, Dmitry (2009) Alcian blue/alizarin red staining of cartilage and bone in mouse. Cold Spring Harbor Protocols, 3: . doi:10.1101/pdb.prot5170

Author Ovchinnikov, Dmitry
Title Alcian blue/alizarin red staining of cartilage and bone in mouse
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1940-3402
Publication date 2009-03
Sub-type Article (original research)
DOI 10.1101/pdb.prot5170
Issue 3
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract The vertebrate skeleton forms by endochondral and intramembranous bone formation. During endochondral bone formation, mesenchyme condensations give rise to cartilages that are eventually replaced by bone. However, there are some permanent cartilages that do not ossify, such as the cartilage of the trachea and articular cartilage of the joints, and intramembranous bone formation occurs directly without a cartilage template. This article describes a method for simultaneously visualizing cartilage and mineralized tissues (notably bone) in the developing mouse. It makes use of alcian blue and alizarin red stains, and it is best used for later fetal, newborn, and early post-natal stages of development. Neonatal skeletons are especially well suited for this technique.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Protocol # 5170. Adapted from Manipulating the Mouse Embryo, 3rd edition (eds. Nagy et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2003.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Fri, 18 Mar 2011, 13:55:46 EST by Dr Dmitry Ovchinnikov on behalf of Aust Institute for Bioengineering & Nanotechnology