An unusual strain of Haemophilus parasuis that fails to react in a species-specific polymerase chain reaction assay

Turni, Conny and Blackall, Patrick J. (2011) An unusual strain of Haemophilus parasuis that fails to react in a species-specific polymerase chain reaction assay. Journal of Veterinary Diagnositic Investigation, 23 2: 355-358. doi:10.1177/104063871102300228


Author Turni, Conny
Blackall, Patrick J.
Title An unusual strain of Haemophilus parasuis that fails to react in a species-specific polymerase chain reaction assay
Journal name Journal of Veterinary Diagnositic Investigation   Check publisher's open access policy
ISSN 1040-6387
1943-4936
Publication date 2011-03-01
Sub-type Article (original research)
DOI 10.1177/104063871102300228
Volume 23
Issue 2
Start page 355
End page 358
Total pages 4
Place of publication Thousand Oaks, CA, United States of America
Publisher Sage Publications
Collection year 2012
Language eng
Formatted abstract
A total of 30 nasal swabs from pigs preweaned and 11 nasal swabs from sick weaned pigs on a farm in Queensland, Australia, were cultured for the presence of Haemophilus parasuis. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genotyping and indirect hemagglutination and gel diffusion serotyping were performed on the retrieved H. parasuis isolates. A total of 3 genotypes were recognized among the 42 isolates recovered, and 4 representative isolates of each genotype were found to be nontypeable in the Kielstein/Rapp-Gabrielson serotyping scheme. A total of 20 of the 22 isolates of genotype 1 did not amplify in the species-specific conventional PCR number 1 (cPCR1) based on the 16S ribosomal RNA (rRNA) gene but did give the expected PCR amplicon in 2 other species-specific PCR assays, one of which is also based on the 16S rRNA gene. Nine selected isolates representing all genotypes, both positive and negative in the cPCR1, were sequenced, and all showed a 4-base mutation occurring at the forward primer annealing site. The quadruple base pair substitution from GTGG to TGTT near the 3' end of the forward primer sequence may explain the failure of amplification. Diagnostic laboratories should be aware that such failures can occur and should consider having an alternative PCR available to confirm negative results or, alternatively, use phenotypic characteristics for the identification of suspect H. parasuis isolates.
Keyword Glässer disease
Haemophilus parasuis
Polymerase chain reaction
16S ribosomal RNA gene
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Fri, 18 Mar 2011, 21:17:00 EST by Samantha Richards on behalf of Qld Alliance for Agriculture and Food Innovation