The dominant 55kDa allergen of the subtropical Bahia grass (Paspalum notatum) pollen is a group 13 pollen allergen, Pas n 13

Davies, Janet M., Voskamp, Astrid, Dang, Thanh D., Pettit, Benjamin, Loo, Dorothy, Petersen, Arnd, Hill, Michelle M., Upham, John W., Rolland, Jennifer M. and O'Hehir, Robyn E. (2011) The dominant 55kDa allergen of the subtropical Bahia grass (Paspalum notatum) pollen is a group 13 pollen allergen, Pas n 13. Molecular Immunology, 48 6-7: 931-940. doi:10.1016/j.molimm.2010.12.013


Author Davies, Janet M.
Voskamp, Astrid
Dang, Thanh D.
Pettit, Benjamin
Loo, Dorothy
Petersen, Arnd
Hill, Michelle M.
Upham, John W.
Rolland, Jennifer M.
O'Hehir, Robyn E.
Title The dominant 55kDa allergen of the subtropical Bahia grass (Paspalum notatum) pollen is a group 13 pollen allergen, Pas n 13
Formatted title
The dominant 55kDa allergen of the subtropical Bahia grass (Paspalum notatum) pollen is a group 13 pollen allergen, Pas n 13
Journal name Molecular Immunology   Check publisher's open access policy
ISSN 0161-5890
1872-9142
Publication date 2011-03
Sub-type Article (original research)
DOI 10.1016/j.molimm.2010.12.013
Volume 48
Issue 6-7
Start page 931
End page 940
Total pages 10
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2012
Language eng
Formatted abstract
Bahia grass, Paspalum notatum, is an important pollen allergen source with a long season of pollination and wide distribution in subtropical and temperate regions. We aimed to characterize the 55 kDa allergen of Bahia grass pollen (BaGP) and ascertain its clinical importance. BaGP extract was separated by 2D-PAGE and immunoblotted with serum IgE of a grass pollen-allergic patient. The amino-terminal protein sequence of the predominant allergen isoform at 55 kDa had similarity with the group 13 allergens of Timothy grass and maize pollen, Phl p 13 and Zea m 13. Four sequences obtained by rapid amplification of the allergen cDNA ends represented multiple isoforms of Pas n 13. The predicted full length cDNA for Pas n 13 encoded a 423 amino acid glycoprotein including a signal peptide of 28 residues and with a predicted pI of 7.0. Tandem mass spectrometry of tryptic peptides of 2D gel spots identified peptides specific to the deduced amino acid sequence for each of the four Pas n 13 cDNA, representing 47% of the predicted mature protein sequence of Pas n 13. There was 80.6% and 72.6% amino acid identity with Zea m 13 and Phl p 13, respectively. Reactivity with a Phl p 13-specific monoclonal antibody AF6 supported designation of this allergen as Pas n 13. The allergen was purified from BaGP extract by ammonium sulphate precipitation, hydrophobic interaction and size exclusion chromatography. Purified Pas n 13 reacted with serum IgE of 34 of 71 (48%) grass pollen-allergic patients and specifically inhibited IgE reactivity with the 55 kDa band of BaGP for two grass pollen-allergic donors. Four isoforms of Pas n 13 from pI 6.3–7.8 had IgE-reactivity with grass pollen allergic sera. The allergenic activity of purified Pas n 13 was demonstrated by activation of basophils from whole blood of three grass pollen-allergic donors tested but not control donors. Pas n 13 is thus a clinically relevant pollen allergen of the subtropical Bahia grass likely to be important in eliciting seasonal allergic rhinitis and asthma in grass pollen-allergic patients.
Keyword Grass pollen allergens
Group 13 pollen allergen
Polygalacturonase
Bahia grass pollen
Pas n 13
IgE
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 26 January 2011.

 
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Created: Fri, 18 Mar 2011, 11:02:00 EST by Dr Janet Davies on behalf of School of Medicine