Immunological techniques: ELISA, flow cytometry, and immunohistochemistry

Ford, Pauline J. (2010) Immunological techniques: ELISA, flow cytometry, and immunohistochemistry. Methods in Molecular Biology, 666 327-343. doi:10.1007/978-1-60761-820-1_20

Author Ford, Pauline J.
Title Immunological techniques: ELISA, flow cytometry, and immunohistochemistry
Journal name Methods in Molecular Biology   Check publisher's open access policy
ISSN 1940-6029
ISBN 978-1-60761-819-5
Publication date 2010
Sub-type Article (original research)
DOI 10.1007/978-1-60761-820-1_20
Volume 666
Start page 327
End page 343
Total pages 17
Place of publication United States
Publisher Humana Press, Inc.
Collection year 2011
Language eng
Abstract Techniques to analyze the host immune response elicited by the presence of oral microorganisms and their products are central to our understanding of the local and systemic effects of oral diseases. This immune response has been extensively investigated for periodontal disease. The local response may result in lesions involving the gingival tissues and depending upon host susceptibility and microbial virulence may lead to local tissue destruction. More recently, however, the importance of the systemic inflammatory and immune response to oral organisms has been recognized. These systemic responses have been associated with an increased risk for cardiovascular disease, diabetes, and preterm low birth weight. A number of techniques are used extensively by researchers investigating humoral and cellular immune responses to oral organisms both in local oral tissues and fluids and systemically in peripheral blood. These are enzyme-linked immunosorbent assay (ELISA) to quantify specific antibody and cytokines in serum, gingival crevicular fluid (GCF), and saliva; characterization of T cells from peripheral blood and gingival tissues using flow cytometry; and immunohistological analysis of the inflammatory cell infiltrate in gingival tissues.
Keyword Antibodies
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Dentistry Publications
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Created: Thu, 17 Mar 2011, 12:53:43 EST by Pauline Ford on behalf of School of Dentistry