Effect of unsaturated bonds in the sn-2 acyl chain of phosphatidylcholine on the membrane-damaging action of Clostridium perfringens alpha-toxin toward liposomes

Nagahama, Masahiro, Otsuka, Akiko, Oda, Masataka, Singh, Rajesh K., Ziora, Zyta M., Imagawa, Hiroshi, Nishizawa, Mugio and Sakurai, Jun (2007) Effect of unsaturated bonds in the sn-2 acyl chain of phosphatidylcholine on the membrane-damaging action of Clostridium perfringens alpha-toxin toward liposomes. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1768 11: 2940-2945. doi:10.1016/j.bbamem.2007.08.016


Author Nagahama, Masahiro
Otsuka, Akiko
Oda, Masataka
Singh, Rajesh K.
Ziora, Zyta M.
Imagawa, Hiroshi
Nishizawa, Mugio
Sakurai, Jun
Title Effect of unsaturated bonds in the sn-2 acyl chain of phosphatidylcholine on the membrane-damaging action of Clostridium perfringens alpha-toxin toward liposomes
Formatted title
Effect of unsaturated bonds in the sn-2 acyl chain of phosphatidylcholine on the membrane-damaging action of Clostridium perfringens alpha-toxin toward liposomes
Journal name Biochimica et Biophysica Acta (BBA) - Biomembranes   Check publisher's open access policy
ISSN 0005-2736
Publication date 2007-11
Sub-type Article (original research)
DOI 10.1016/j.bbamem.2007.08.016
Volume 1768
Issue 11
Start page 2940
End page 2945
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
Clostridium perfringens alpha-toxin degrades phosphatidylcholine (PC) in the bilayer of liposomes and destroys the membrane. The effect of the type and position of unsaturation in the fatty acyl chain of PC (18:0/18:1 PC) synthesized on the toxin-induced leakage of carboxyfluorescein (CF) from PC liposomes was examined. Differential scanning calorimetry showed that the phase transition temperature (Tm) was minimal when the triple bond was positioned at C (9) in the sn-2 acyl chain. The toxin-induced CF leakage decreased with the migration of the bond from C (9) to either end of the acyl chain in PC. The PC containing the cis-double bond had a similar Tm to that with the triple bond, but a lower value than the PC containing the trans-double bond. Furthermore, the toxin-induced leakage from liposomes composed of PC containing the cis-double bond resembled that with PC having the triple bond and was greater than that from liposomes with PC having the trans-double bond. The binding of a H148G mutant to PC liposomes showed a reciprocal relationship in terms of the Tm value of PC containing the triple bond. These results indicate that the toxin-induced membrane damage is closely related to membrane fluidity in liposomes.
Keyword Clostridium perfringens
Alpha-toxin
Membrane fluidity
Phase transition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 17 Mar 2011, 10:34:40 EST by Susan Allen on behalf of Institute for Molecular Bioscience