Probing expansin action using cellulose/hemicellulose composites

Whitney, Sarah E. C., Gidley, Mike J. and McQueen-Mason, Simon J. (2000) Probing expansin action using cellulose/hemicellulose composites. Plant Journal, 22 4: 327-334. doi:10.1046/j.1365-313x.2000.00742.x


Author Whitney, Sarah E. C.
Gidley, Mike J.
McQueen-Mason, Simon J.
Title Probing expansin action using cellulose/hemicellulose composites
Journal name Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2000-05
Sub-type Article (original research)
DOI 10.1046/j.1365-313x.2000.00742.x
Volume 22
Issue 4
Start page 327
End page 334
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract Cellulose-based composite materials containing xyloglucans or mannan-based polysaccharides have been shown to possess organisational features with many characteristics similar to primary plant cell walls. We have tested the effects of a typical α-expansin (CsExp1) on these composites using two different mechanical assays. We show that CsExp1 induces very rapid extension in composites containing tamarind xyloglucan under constant load. In contrast, expansin treatment had no effect in constant load extension assays using cellulose-only materials or in those carried out on composites containing glucomannan or galactomannan. We show that the effect of expansins is much smaller on composites made with short chain length xyloglucans than on those containing longer chains. In uniaxial extension tests we found that expansin could double the total extension (before failure) in xyloglucan composites and that the effects were again lower in composites containing shorter xyloglucans. We found no effect of expansin on uniaxial extensions with glucomannan or galactomannan. However, a significant effect of expansin on the uniaxial extension behaviour of cellulose-only material was observed. These experiments suggest that the target of CsExp1 in cell walls is probably the cellulose xyloglucan matrix, but that other (1-4) β-glucan to (1-4) β-glucan hydrogen bonded contacts can also serve as substrates.
Keyword Cell wall extension
Cucumber hypocotyls
Cellulose
Xyloglucan
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 25 December 2001.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 102 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 16 Mar 2011, 20:39:40 EST