Effect of isolation procedures on the molecular composition and physical properties of Eucheuma cottonii carrageenan

Hoffmann, R. A., Gidley, M. J., Cooke, D. and Frith, W. J. (1995). Effect of isolation procedures on the molecular composition and physical properties of Eucheuma cottonii carrageenan. In: Conference on Food Hydrocolloids (OHIO 94), Columbus, OH, United States, (281-289). 6-10 September 1994.

Author Hoffmann, R. A.
Gidley, M. J.
Cooke, D.
Frith, W. J.
Title of paper Effect of isolation procedures on the molecular composition and physical properties of Eucheuma cottonii carrageenan
Conference name Conference on Food Hydrocolloids (OHIO 94)
Conference location Columbus, OH, United States
Conference dates 6-10 September 1994
Journal name Food Hydrocolloids   Check publisher's open access policy
Place of Publication Amsterdam, Netherlands
Publisher Elsevier
Publication Year 1995
Sub-type Fully published paper
ISSN 0268-005X
1873-7137
Volume 9
Issue 4
Start page 281
End page 289
Total pages 8
Language eng
Abstract/Summary Direct treatment of Eucheuma cottonii with hot potassium hydroxide leads to a solid residue (alkali treated weed) which was found to contain predominantly carrageenan and cellulose. Following dispersion and heating iri water, separation into polymer-containing solution and an insoluble particulate phase was readily achieved. Chemical analysis of solubilized polymers showed that the predominant carrageenan present in native weed was a mu/kappa-carrageenan which was readily converted into kappa with hot alkali. The particulate phase from both materials was predominantly cellulose; characterization by X-ray diffraction showed the presence of partial cellulose type I crystallinity. Solid state C-13-NMR spectra showed that this crystalline fraction was similar to 53% of the total cellulose and was comprised of similar to 40% type I alpha and similar to 60% type I beta. The cellulosic part seemed unaffected by the hot alkali treatment. A carrageenan type polysaccharide was found associated with the cellulosic fraction; which underwent the same conversion as the mu/kappa carrageenan on alkali treatment. Further refining of solubilized carrageenan by isopropyl alcohol precipitation, gel pressing or freeze-drying, resulted in only minor differences in chemical composition. Hot solution viscosity and gelation properties in the presence of potassium chloride were measured showing no significant differences in gelling behaviour between alkali treated weed and the different carrageenan isolates but a higher solution viscosity for the alkali treated weed.
Keyword NATIVE CELLULOSES
DOMAIN MODEL
C-13 NMR
HELICES
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: Centre for Nutrition and Food Sciences Publications
 
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Created: Wed, 16 Mar 2011, 20:34:50 EST