A xyloglucan-oligosaccharide-specific α-d-xylosidase or exo-oligoxyloglucan-α-xylohydrolase from germinated nasturtium (Tropaeolum majus L.) seeds - Purification, properties and its interaction with a xyloglucan-specific eneto-(1→4)-β-d-glucanase and

Fanutti, Cristina, Gidley, Michael J. and Reid, J.S. Grant (1991) A xyloglucan-oligosaccharide-specific α-d-xylosidase or exo-oligoxyloglucan-α-xylohydrolase from germinated nasturtium (Tropaeolum majus L.) seeds - Purification, properties and its interaction with a xyloglucan-specific eneto-(1→4)-β-d-glucanase and other hydrolases during storage-xyloglucan mobilisation. Planta, 184 1: 137-147. doi:10.1007/BF00208247


Author Fanutti, Cristina
Gidley, Michael J.
Reid, J.S. Grant
Title A xyloglucan-oligosaccharide-specific α-d-xylosidase or exo-oligoxyloglucan-α-xylohydrolase from germinated nasturtium (Tropaeolum majus L.) seeds - Purification, properties and its interaction with a xyloglucan-specific eneto-(1→4)-β-d-glucanase and other hydrolases during storage-xyloglucan mobilisation
Journal name Planta   Check publisher's open access policy
ISSN 0032-0935
1432-2048
Publication date 1991
Sub-type Article (original research)
DOI 10.1007/BF00208247
Volume 184
Issue 1
Start page 137
End page 147
Total pages 11
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
The α-xylosidase which is involved in the postgerminative mobilisation of xyloglucan in nasturtium seed cotyledons has now been purified to apparent homogeneity by a facile procedure involving lectin affinity chromatography. The purified enzyme, a glycoprotein, moved as a single band (apparent molecular weight 85000) on sodium dodecyl sulphate-gel electrophoresis, whilst isoelectric focusing gave a number of enzymatically active protein bands spanning the range pI = 5.0 to 7.1 (maximum activity at pI = 6.1). The enzyme did not hydrolyse the simple α-xylosides p-nitrophenyl-α-d-xylopyranoside and woprimeverose (α-d-Xyl(1→6)-d-Glc), or polymeric tamarind-seed xyloglucan. It released xylose from a complex mixture of oligosaccharides produced by exhaustive hydrolysis of tamarind seed xyloglucan using the xyloglucan-specific endo-(1→4)-β-d-glucanase from germinated nasturtium seeds (M. Edwards et al. 1986, J. Biol. Chem., 261. 9489-9494). The three xyloglucan oligosaccharides of lowest molecular size were purified from this mixture and were shown by 1H-nuclear magnetic resonance (1H-NMR) and enzymatic analysis to have the structures:
Keyword Cell wall
Endo-(1-]4)-beta-D-glucanase (xyloglucan-specific)
Storage polysaccharide (seed)
Tropaeolum (xyloglucan mobilization)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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