Chromatographic analysis of diverse fruit components using HPLC and UPLC

D'Arcy, B., Epriliati, I., Gidley, M.J. and Kerven, G. (2010) Chromatographic analysis of diverse fruit components using HPLC and UPLC. Analytical Methods, 2 10: 1606-1613. doi:10.1039/c0ay00244e


Author D'Arcy, B.
Epriliati, I.
Gidley, M.J.
Kerven, G.
Title Chromatographic analysis of diverse fruit components using HPLC and UPLC
Journal name Analytical Methods   Check publisher's open access policy
ISSN 1759-9660
1759-9679
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1039/c0ay00244e
Volume 2
Issue 10
Start page 1606
End page 1613
Total pages 8
Place of publication London, U.K.
Publisher Royal Society of Chemistry
Collection year 2011
Language eng
Abstract The nutritional properties of fruits are due to the combined action of many categories of molecules. Studies on molecular nutrition, therefore, requires analytical methods that can cope with diverse molecular groups, including metabolites that may be currently unknown. Fractionation and identification protocols are crucial to validate identified compounds, and can be adapted from methodologies developed for metabolome studies. Pectin-related problems during fractionation of samples from ripe fruit products, both undigested and after digestion, as well as samples from in vitrocell culture bioassay were addressed, and chromatographic separations of target compounds (carotenoids, phenolics, sugars, and organic acids) were developed based on RPchromatography using HPLC and UPLC systems. The final method comprised of a chloroform–methanol–water (2 : 1 : 3) extraction to simultaneously extract as many polar and nonpolar compounds as possible, followed by chromatographic analysis using (a) a HPLC-ELSD-UV system with a Prevail Carbohydrate column for sugar analysis, (b) Prevail Organic Acid column for organic acid analysis, (c) LC-MS ESI of eluants from a XTerra C-18 column for phenolics and (d) UPLC-PDA systems in a C-18-Bridged Ethane in Hybrid matrix column for carotenoids and other hydrophobic components. Acetonitrile-based mobile phases can be used to run all chromatographic systems developed. © Royal Society of Chemistry 2011
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
Centre for Nutrition and Food Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 16 Mar 2011, 20:31:45 EST