A new family of oligosaccharides from the xyloglucan of Hymenaea courbaril L. (Leguminosae) cotyledons

Buckeridge, M.S., Crombie, H.J., Mendes, C.J.M., Reid, J.S.G., Gidley, M.J. and Vieira, C.C.J. (1997) A new family of oligosaccharides from the xyloglucan of Hymenaea courbaril L. (Leguminosae) cotyledons. Carbohydrate Research, 303 2: 233-237. doi:10.1016/S0008-6215(97)00161-4

Author Buckeridge, M.S.
Crombie, H.J.
Mendes, C.J.M.
Reid, J.S.G.
Gidley, M.J.
Vieira, C.C.J.
Title A new family of oligosaccharides from the xyloglucan of Hymenaea courbaril L. (Leguminosae) cotyledons
Journal name Carbohydrate Research   Check publisher's open access policy
ISSN 0008-6215
Publication date 1997-09
Sub-type Article (original research)
DOI 10.1016/S0008-6215(97)00161-4
Volume 303
Issue 2
Start page 233
End page 237
Total pages 5
Place of publication Oxford, United Kingdom
Publisher Pergamon
Language eng
Formatted abstract
The xyloglucan from cotyledons of Hymenaea courbaril was hydrolysed with endo-(1,4)β-D-glucanase (cellulase) and analysed by TLC and HPAEC. The limit digest was different from those obtained from xyloglucans of Tamarindus indica and Copaifera langsdorffii. On treatment with nasturtium β-galactosidase, two main oligosaccharides were detected by TLC and HPAEC. Using a process of enzymatic sequencing involving alternate treatments with a pure xyloglucan oligosaccharide-specific α-xylosidase, and a pure β-glucosidase, both from nasturtium, their structures were deduced to be XXXG and a new oligosaccharide XXXXG. These structures were confirmed by 1H NMR. The relative proportions of XXXG and XXXXG indicate that approximately half of the subunits in Hymenaea xyloglucan are based on the new oligosaccharides. In the native polymer the XXXXG subunits are likely to carry galactosyl substituents in varying proportions, since cellulase hydrolysates contained many bands which were converted to XXXXG on hydrolysis with nasturtium β-galactosidase. Although no comparative studies on the physico-chemical properties of Hymenaea courbaril xyloglucan have yet been performed, our results indicate that this polymer is less interactive with iodine when compared with T. indica and C. langsdorffii xyloglucans, suggesting that changes in conformation may occur due to the presence of XXXXG.
Keyword Xyloglucan
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
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Created: Wed, 16 Mar 2011, 20:30:18 EST