Histology of waxflower (Chamelaucium spp.) flower infection by Botrytis cinerea

Dinh, S. Q., Joyce, D. C., Irving, D. E. and Wearing, A. H. (2011) Histology of waxflower (Chamelaucium spp.) flower infection by Botrytis cinerea. Plant Pathology, 60 2: 278-287. doi:10.1111/j.1365-3059.2010.02347.x


Author Dinh, S. Q.
Joyce, D. C.
Irving, D. E.
Wearing, A. H.
Title Histology of waxflower (Chamelaucium spp.) flower infection by Botrytis cinerea
Formatted title
Histology of waxflower (Chamelaucium spp.) flower infection by Botrytis cinerea
Journal name Plant Pathology   Check publisher's open access policy
ISSN 0032-0862
Publication date 2011-04
Year available 2010
Sub-type Article (original research)
DOI 10.1111/j.1365-3059.2010.02347.x
Volume 60
Issue 2
Start page 278
End page 287
Total pages 10
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Collection year 2011
Language eng
Abstract Botrytis cinerea infects waxflower (Chamelaucium spp.) flowers and can induce them to abscise from their petioles before disease becomes evident. Botrytis cinerea infection of flowers was studied on two waxflower cultivars by light and electron microscopy. Pot-grown waxflower flowers were harvested, inoculated with aqueous suspensions of B. cinerea conidia, incubated at 20-22°C and >95% RH and examined within 96h post-inoculation (hpi). Conidial germination on petals started 4hpi, penetration via germ tube tips was 6hpi and protoappressoria were formed 8hpi. Germination on petals approximately doubled every 4-6h to 18hpi. Conidial germination was ca. 50% at 22-24hpi. Botrytis cinerea infected most waxflower flower organs, including petals, anthers and filaments, stigma and hypanthium, within 24hpi. Hyaline and lobate appressoria were observed 36hpi. Infection cushions on stamen bases were formed 36hpi by saprophytic hyphae that originated from anthers. This infection process can give rise to tan-coloured symptoms typical of botrytis disease that radiate from this part of the flower. Subcuticular hyphae were present at high density near stamen bases and evidently resulted from multiple penetrations from single infection cushions. The subcuticular hyphae grew within the hypanthium and towards the centre of the floral tube. When flower abscission occurred, floral tube tissues close to the abscission zone remained uninfected. This observation infers possible transmission of a signal (e.g. ethylene) upon B. cinerea infection. Thus, B. cinerea causes flower abscission apparently as a defence response. © 2010 The Authors. Plant Pathology © 2010 BSPP.
Keyword Abscission
Appressoria
Conidial germination
Histopathology
Postharvest disease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 26 AUG 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Created: Tue, 15 Mar 2011, 15:23:06 EST by Daryl Joyce on behalf of Centre for Native Floriculture