Analysis of human V alpha 24(+) CD4(+) NKT cells activated by alpha-glycosylceramide-pulsed monocyte-derived dendritic cells

Takahashi, T., Nieda, M., Koezuka, Y., Nicol, A., Porcelli, S.A., Ishikawa, Y., Tadokoro, K., Hirai, H. and Juji, T. (2000) Analysis of human V alpha 24(+) CD4(+) NKT cells activated by alpha-glycosylceramide-pulsed monocyte-derived dendritic cells. Journal of Immunology, 164 9: 4458-4464.

Author Takahashi, T.
Nieda, M.
Koezuka, Y.
Nicol, A.
Porcelli, S.A.
Ishikawa, Y.
Tadokoro, K.
Hirai, H.
Juji, T.
Title Analysis of human V alpha 24(+) CD4(+) NKT cells activated by alpha-glycosylceramide-pulsed monocyte-derived dendritic cells
Formatted title
Analysis of human Vα24+ CD4+ NKT cells activated by α- glycosylceramide-pulsed monocyte-derived dendritic cells
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2000-05
Sub-type Article (original research)
Volume 164
Issue 9
Start page 4458
End page 4464
Total pages 7
Place of publication Bethesda, MD, United States
Publisher American Association of Immunologists
Language eng
Formatted abstract
Human Vα24+ NKT cells with an invariant TCR (Vα24-JαQ) have been shown to be specifically activated by synthetic glycolipids such as α- galactosylceramide and α-glucosylceramide in a CD1d-restricted and Vα24 TCR-mediated manner. We recently characterized Vα24+ CD4- CDS- double negative (DN) NKT cells using α-galactosylceramide-pulsed monocyte-derived dendritic cells. Here, we compare Vα24+ CD4+ NKT cells with human Vα24+ DN NKT cells from the same donor using α-galactosylceramide-pulsed monocyte- derived dendritic cells. Human Vα24+ CD4+ NKT cells were phenotypically and functionally similar to the human Vα24+ DN NKT cells characterized previously. Both of them use Vα24-J∅q-Vβ11 TCR and express CD161 (NKRP1A), but not the other NK receptors tested so far. They also produce cytokines such as IL-4 and IFN-γ, and, in regard to IL-4 production, Vα24+ CD4+ NKT cells produce more IL-4 than Vα24+ DN NKT cells. The cells exhibit marked cytotoxic activity against the U937 tumor cell line, but not against the NK target cell line, K562. Although at least some of the factors responsible for the stimulation of Vα24+ NKT cells have been clarified, little is known regarding the killing phase of these cells. Here we show that the cytotoxic activity of Vα24+ NKT cells against U937 cells is mediated mainly through the perforin pathway and that ICAM-1/LFA-1 as well as CD44/hyaluronic acid interactions are important for the effector phase of Vα24+ NKT cell- mediated cytotoxicity against U937 cells.
Keyword Killer T cells
TCR alpha
Antigen receptor
Expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 122 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 15 Mar 2011, 11:31:21 EST