Optimal cryopreservation of human umbilical cord blood

Donaldson, C., Armitage, W.J., Denning-Kendall, P.A., Nicol, A.J., Bradley, B.A. and Hows, J.M. (1996) Optimal cryopreservation of human umbilical cord blood. Bone Marrow Transplantation, 18 4: 725-731.

Author Donaldson, C.
Armitage, W.J.
Denning-Kendall, P.A.
Nicol, A.J.
Bradley, B.A.
Hows, J.M.
Title Optimal cryopreservation of human umbilical cord blood
Journal name Bone Marrow Transplantation   Check publisher's open access policy
ISSN 0268-3369
Publication date 1996-10
Sub-type Article (original research)
Volume 18
Issue 4
Start page 725
End page 731
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
Cryopreservation techniques for umbilical cord blood (UCB) have been based on methods established for marrow (BM) and peripheral blood progenitor cells (PBPC) with varying degrees of success. The aim of this study was to optimise cryopreservation of UCB haemopoietic cells based on sound cryopreservation principles, UCB samples were cryopreserved with different combinations of DMSO and hydroxyethyl starch (HES) by a variety of freezing protocols. After cooling at 1°C/min in solutions containing 4% HES and various concentrations of DMSO there was a dramatic fall in CD34+ recovery from 85.4% (s.d. 28.4) to 12.2% (s.d. 10.0) as DMSO concentration was reduced from 5 to 2.5%. Varying HES concentration in solutions containing 5% DMSO did not have a significant effect on CD34+ cell recovery. Increasing cooling rate from 1 to 10°C/min significantly reduced CD34+ recovery (P < 0.0001) while increasing DMSO concentration up to 10% had little effect (P = 0.8, two-way ANOVA). Good recovery of UCB CD34+ cells can be achieved with 5-10% DMSO at a controlled cooling rate of 1°C/min. There was a significant difference (P < 0.0001) in the apparent recovery of CD34+ cells between paired aliquots thawed in the presence (recovery = 76.8%, s.d. 26.0) and absence (32.5%, s.d. 18.7) of DNase. In conclusion, conditions for cryopreserving UCB for clinical banking that yield optimal recovery of CD34+ cells have been established.
Keyword Cord blood
Progenitor cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Tue, 15 Mar 2011, 11:30:23 EST