GATA-1 associates with and inhibits p53

Trainor, Cecelia D., Mas, Caroline, Archambault, Patrick, Di Lello, Paola and Omichinski, James G. (2009) GATA-1 associates with and inhibits p53. Blood, 114 1: 165-173. doi:10.1182/blood-2008-10-180489

Author Trainor, Cecelia D.
Mas, Caroline
Archambault, Patrick
Di Lello, Paola
Omichinski, James G.
Title GATA-1 associates with and inhibits p53
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
Publication date 2009-07-02
Sub-type Article (original research)
DOI 10.1182/blood-2008-10-180489
Volume 114
Issue 1
Start page 165
End page 173
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Language eng
Abstract In addition to orchestrating the expression of all erythroid-specific genes, GATA-1 controls the growth, differentiation, and survival of the erythroid lineage through the regulation of genes that manipulate the cell cycle and apoptosis. The stages of mammalian erythropoiesis include global gene inactivation, nuclear condensation, and enucleation to yield circulating erythrocytes, and some of the genes whose expression are altered by GATA-1 during this process are members of the p53 pathway. In this study, we demonstrate a specific in vitro interaction between the transactivation domain of p53 (p53TAD) and a segment of the GATA-1 DNA-binding domain that includes the carboxyl-terminal zinc-finger domain.We also show by immunoprecipitation that the native GATA-1 and p53 interact in erythroid cells and that activation of p53-responsive promoters in an erythroid cell line can be inhibited by the overexpression of GATA-1. Mutational analysis reveals that GATA-1 inhibition of p53 minimally requires the segment of the GATA-1 DNA-binding domain that interacts with p53TAD. This inhibition is reciprocal, as the activation of a GATA-1-responsive promoter can be inhibited by p53. Based on these findings, we conclude that inhibition of the p53 pathway by GATA-1 may be essential for erythroid cell development and survival.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 14 Mar 2011, 11:35:37 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging