Differential phenotypic characteristics of heterogeneous cell population in the rabbit periosteum

Youn, Inchan, Shu, Jun-Kyo Francis, Nauman, Eric A. and Jones, Deryk G. (2005) Differential phenotypic characteristics of heterogeneous cell population in the rabbit periosteum. Acta Orthopaedica, 76 3: 442-450. doi:10.1080/1743670510041367


Author Youn, Inchan
Shu, Jun-Kyo Francis
Nauman, Eric A.
Jones, Deryk G.
Title Differential phenotypic characteristics of heterogeneous cell population in the rabbit periosteum
Journal name Acta Orthopaedica   Check publisher's open access policy
ISSN 1745-3674
1745-3682
Publication date 2005-06
Sub-type Article (original research)
DOI 10.1080/1743670510041367
Open Access Status DOI
Volume 76
Issue 3
Start page 442
End page 450
Total pages 9
Place of publication Colchester, United Kingdom
Publisher Taylor & Francis
Language eng
Formatted abstract
Background: Periosteum and periosteum-derived progenitor cells have demonstrated the potential for stimulative applications in repair of various musculoskeletal tissues. It has been found that the periosteum contains mesenchymal progenitor cells that are capable of differentiating into either osteoblasts or chondrocytes, depending on the culture conditions. Anatomically, the periosteum is a heterogeneous multilayered membrane, consisting of an outer fibrous and an inner cambium layer. The present study was designed to elucidate the phenotypic characteristics of fibrous and cambium layer cells in vitro.

Methods: Using a sequential enzymatic digestion method, fibrous and cambium layer cells were harvested separately from periosteum-bone explants of the proximal tibia of 6-month-old New Zealand White rabbits.

Results:
We found that the cells from each layer showed distinct phenotypic characteristics in a primary monolayer culture system. Specifically, the cambium cells demonstrated higher osteogenic characteristics (higher alkaline phosphatase and osteocalcin levels) than the fibrous cells. However, these differences diminished with time in vitro. Interpretation Our findings suggest that the periosteum has phenotypically distinct heterogeneous cell populations. Care must be taken in order to identify and distinguish the intrinsic phenotypes of the heterogeneous periosteum-derived cell types in vitro.
Keyword Full-thickness defects
In-vitro
Bone
Grafts
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Mon, 14 Mar 2011, 09:20:02 EST