Chronic perioperative steroids and colonic anastomotic healing in rats

Del Rio, Juan V., Beck, David E. and Opelka, Frank G. (1996) Chronic perioperative steroids and colonic anastomotic healing in rats. Journal of Surgical Research, 66 2: 138-142. doi:10.1006/jsre.1996.0385

Author Del Rio, Juan V.
Beck, David E.
Opelka, Frank G.
Title Chronic perioperative steroids and colonic anastomotic healing in rats
Journal name Journal of Surgical Research   Check publisher's open access policy
ISSN 0022-4804
Publication date 1996-12
Sub-type Article (original research)
DOI 10.1006/jsre.1996.0385
Volume 66
Issue 2
Start page 138
End page 142
Total pages 4
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
Objective: To develop a rat model of long-term high-dose perioperativesteroids and to evaluate the effects of these steroids on a colonic anastomosis in this model. Design: Prospective randomized. Methods: Twenty-six male Sprague–Dawley rats, weighing 270 to 330 g, were randomized into two groups. The first group (steroid group) (13 rats) received a time-release drug pellet (200 mg cortisone acetate in a 60-day release form) placed in the subcutaneous tissue of the posterior neck for an average daily dose of 3.3 mg. The second group (control group) (13 rats) received a placebo. At 6 weeks, blood cortisol levels were measured, and a colonic anastomosis was performed 2.5 cm distal to the cecum. Steroid group animals also received cortisone acetate (5 mg intramuscularly) immediately before surgery. Colonic bursting strength (mmHg) was measured at the anastomosis site and in the normal distal left colon using a saline infusion system at 8 and 12 days postoperatively. Results: Blood cortisol levels were significantly higher in the rats in the steroid group than in the rats in the control group. The anastomotic bursting strength was significantly lower in the steroid group at Days 8 and 12. The bursting pressure of the unoperated left colon was not significantly different when the groups were compared. Also, in the steroid group, healing of the pellet insertion wounds in the neck was impaired. Conclusion: The time-release drug pellet is a reliable method of administering long-term steroids. Long-term perioperativesteroids impaired colonicanastomotichealing, while normal tissue strength (left colon) was not significantly changed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 14 Mar 2011, 08:47:22 EST