A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

Iturbe-Ormaetxe, I, Woolfit, M, Rances, E, Duplouy, A and O'Neill, SL (2011) A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing. Journal of Microbiological Methods, 84 1: 134-136. doi:10.1016/j.mimet.2010.10.019


Author Iturbe-Ormaetxe, I
Woolfit, M
Rances, E
Duplouy, A
O'Neill, SL
Title A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing
Formatted title
A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing
Journal name Journal of Microbiological Methods   Check publisher's open access policy
ISSN 0167-7012
1872-8359
Publication date 2011-01
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.mimet.2010.10.019
Volume 84
Issue 1
Start page 134
End page 136
Total pages 3
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.
© 2010 Elsevier B.V.
Keyword Wolbachia pipientis
Purification
Genomics
Symbiosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 31 October 2010. Published under Notes.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Sun, 13 Mar 2011, 00:11:17 EST