A coproantigen diagnostic test for Strongyloides infection

Sykes, Alex M. and McCarthy, James S. (2011) A coproantigen diagnostic test for Strongyloides infection. PLoS Neglected Tropical Diseases, 5 2: 1-8. doi:10.1371/journal.pntd.0000955

Author Sykes, Alex M.
McCarthy, James S.
Title A coproantigen diagnostic test for Strongyloides infection
Journal name PLoS Neglected Tropical Diseases   Check publisher's open access policy
ISSN 1935-2727
Publication date 2011-02
Sub-type Article (original research)
DOI 10.1371/journal.pntd.0000955
Open Access Status DOI
Volume 5
Issue 2
Start page 1
End page 8
Total pages 8
Place of publication San Francisco CA , United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
Accurate diagnosis of infection with the parasite Strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. Even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. In this manuscript we describe the development of a coproantigen ELISA for diagnosis of infection. Polyclonal rabbit antiserum was raised against Strongyloides ratti excretory/secretory (E/S) antigen and utilized to develop an antigen capture ELISA. The assay enabled detection of subpatent rodent S. ratti and human S. stercoralis infection. No cross-reactivity was observed with purified E/S from Schistosoma japonicum, the  hookworms Ancylostoma caninum, A. ceylanicum, nor with fecal samples collected from rodents harboring Trichuris muris or S. mansoni infection. Strongyloides coproantigens that appear stable when frozen as formalin-extracted fecal supernatants stored at −20°C remained positive up to 270 days of storage, whereas supernatants stored at 4°C tested negative. These results indicate that diagnosis of human strongyloidiasis by detection of coproantigen is an approach worthy of further development.
Keyword Linked-immunosorbent-assay
Antigen capture ELISA
Fecal specimens
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Sun, 13 Mar 2011, 00:05:11 EST