Label-free protein detection via gold nanoparticles and localized surface plasmon resonance

Zhu, S. L., Zhang, J. B., Yue, Lanry Y. L., Hartono, D. and Liu, A. Q. (2009). Label-free protein detection via gold nanoparticles and localized surface plasmon resonance. In: Selin Teo, A. Q. Liu, H. Li and B. Tarik, NEMS/MEMS Technology and Devices. 5th International Conference on Materials for Advanced Technologies (ICMAT 2009), Singapore, (95-98). 28 June - 3 July 2009. doi:10.4028/www.scientific.net/AMR.74.95

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Author Zhu, S. L.
Zhang, J. B.
Yue, Lanry Y. L.
Hartono, D.
Liu, A. Q.
Title of paper Label-free protein detection via gold nanoparticles and localized surface plasmon resonance
Conference name 5th International Conference on Materials for Advanced Technologies (ICMAT 2009)
Conference location Singapore
Conference dates 28 June - 3 July 2009
Proceedings title NEMS/MEMS Technology and Devices   Check publisher's open access policy
Journal name Advanced Materials Research   Check publisher's open access policy
Place of Publication Stafa-Zurich, Switzerland
Publisher Trans Tech Publications
Publication Year 2009
Sub-type Fully published paper
DOI 10.4028/www.scientific.net/AMR.74.95
Open Access Status File (Author Post-print)
ISBN 978-0-87849-321-0
ISSN 1022-6680
Editor Selin Teo
A. Q. Liu
H. Li
B. Tarik
Volume 74
Start page 95
End page 98
Total pages 4
Language eng
Abstract/Summary Protein plays key role in cellular processes and diseases diagnosis [1-3]. This paper reports a label-free detection of proteins through localized surface plasmon resonance (LSPR) and realized Au nanoparticles (AuNPs) immobilized on glass slide. Glass slide is first sonicated with 5% v/v glassware detergent in ultrapure water for 30 mins, and then in ultrapure water for another 30 mins. A 5% v/v aminopropyltriethoxysilane (APTES) solution in ultrapure water is prepared and the glass slide is immersed in the 5% v/v APTES solution for 20 mins. The glass slide is sonicated for 60 mins in ultrapure water. The purpose of the sonication is to remove the multi-layers of APTES which are formed on the glass surface. The silanized glass slide is immersed in AuNPs solution for 40 mins. Finally, the protein solution is added on the surface of the slide. The surface absorption of proteins induces a significant difference of environmental refractive index around the AuNPs. The shift in the wavelength of the LSPR spectra is very sensitive to the change in the surface refractive index of the nanoparticles. The shifts in the LSPR spectra are primarily determined by the volatility and refractive indices of the protein species. In this paper, the LSPR-nanobiosensor is designed and fabricated. The LSPR band responses are measured by a real-time UV–vis spectrometer with a CCD array detector. The response time of the protein–LSPR spectrum is less than 3 seconds and the response is reversible and reproducible.
Keyword Gold nanoparticles
Label-free
Localized surface plasmon resonance
Protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

 
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Created: Fri, 11 Mar 2011, 14:01:34 EST by Dr Shaoli Zhu on behalf of Aust Institute for Bioengineering & Nanotechnology