Antioxidant supplementation reduces skeletal muscle mitochondrial biogenesis

Strobel, Natalie A., Peake, Jonathan M., Matsumoto, Aya, Marsh, Susan A., Coombes, Jeff S. and Wadley, Glenn D. (2011) Antioxidant supplementation reduces skeletal muscle mitochondrial biogenesis. Medicine and Science in Sports and Exercise, 43 6: 1017-1024.


Author Strobel, Natalie A.
Peake, Jonathan M.
Matsumoto, Aya
Marsh, Susan A.
Coombes, Jeff S.
Wadley, Glenn D.
Title Antioxidant supplementation reduces skeletal muscle mitochondrial biogenesis
Journal name Medicine and Science in Sports and Exercise  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2011-06
Sub-type Article
Year available 2010
DOI 10.1249/MSS.0b013e318203afa3
Volume number 43
Issue number 6
ISSN 0195-9131
Start page 1017
End page 1024
Total pages 8
Place of publication United States
Publisher Lippincott Williams & Wilkins
Collection year 2011
Language eng
Formatted abstract PURPOSE:
Exercise increases the production of reactive oxygen species (ROS) in skeletal muscle and athletes often consume antioxidant supplements in the belief they will attenuate ROS related muscle damage and fatigue during exercise. However, exercise-induced ROS may regulate beneficial skeletal muscle adaptations, such as increased mitochondrial biogenesis. We therefore investigated the effects of long-term antioxidant supplementation with vitamin E and α-lipoic acid on changes in markers of mitochondrial biogenesis in the skeletal muscle of exercise-trained and sedentary rats.

METHODS:
Male Wistar rats were divided into four groups: 1) sedentary control diet, 2) sedentary antioxidant diet, 3) exercise control diet and 4) exercise antioxidant diet. Animals ran on a treadmill four days/week at ~70% VO2max for up to 90 min/day for 14 weeks.

RESULTS:
Consistent with augmentation of skeletal muscle mitochondrial biogenesis and antioxidant defenses, following training there were significant increases in PGC-1α mRNA and protein, COX IV and cytochrome C protein abundance, citrate synthase activity, Nfe2l2 and SOD2 protein (P<0.05). Antioxidant supplementation reduced PGC-1α mRNA, PGC-1α and COX IV protein, and citrate synthase enzyme activity (P<0.05) in both sedentary and exercise-trained rats.

CONCLUSION:
Vitamin E and α-lipoic acid supplementation suppresses skeletal muscle mitochondrial biogenesis, regardless of training status.
Keyword α-lipoic acid
Exercise-training
Mitochondria
Nfe2l2
PGC-1α
Vitamin E
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2011 Collection
School of Human Movement Studies Publications
 
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Created: Thu, 10 Mar 2011, 13:36:05 EST by Deborah Noon on behalf of School of Human Movement Studies