Comparison of DNA marker technologies in characterizing plant genome diversity: variability in Chinese sorghums

Yang, W.P., deOliveira, A.C., Godwin, I., Schertz, K. and Bennetzen, J.L. (1996) Comparison of DNA marker technologies in characterizing plant genome diversity: variability in Chinese sorghums. Crop Science, 36 6: 1669-1676.

Author Yang, W.P.
deOliveira, A.C.
Godwin, I.
Schertz, K.
Bennetzen, J.L.
Title Comparison of DNA marker technologies in characterizing plant genome diversity: variability in Chinese sorghums
Journal name Crop Science   Check publisher's open access policy
ISSN 0011-183X
1435-0653
Publication date 1996-11
Sub-type Article (original research)
Volume 36
Issue 6
Start page 1669
End page 1676
Total pages 8
Place of publication Madison, WI, United States
Publisher Crop Science Society of America
Language eng
Abstract Chinese sorghums [Sorghum bicolor (L.) Moench] are reputed to have a narrow genetic base, resulting from infrequent introduction of exotic germplasm into China. We have used several different molecular approaches to evaluate diversity and relatedness in a selection of 34 Chinese sorghums. The results indicated that different DNA marker technologies for germplasm assessment yield comparable results, but that a relatively new technique termed inter-simple sequence repeat amplification (ISSR) was relatively rapid, reproducible, and inexpensive. Extensive diversity was observed within the Chinese sorghums, and all lines could be easily differentiated. Different lines collected from the same locality were found to exhibit particularly high levels of relatedness. Contrary to expectations, improved varieties were found to contain more diversity and to be more different from each other than were the Chinese landraces studied, suggesting recent introduction of non-Chinese germplasm into these improved materials
Keyword Cultivated sorghum
Arbitrary primers
Genetic analysis
Polymorphisms
Evolution
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Thu, 10 Mar 2011, 11:04:00 EST