Effect of ethylene and culture environment on development of papaya nodal cultures

Magdalita, P. M., Godwin, I. D., Drew, R. A. and Adkins, S. W. (1997) Effect of ethylene and culture environment on development of papaya nodal cultures. Plant Cell, Tissue and Organ Culture, 49 2: 93-100. doi:10.1023/A:1005869211684


Author Magdalita, P. M.
Godwin, I. D.
Drew, R. A.
Adkins, S. W.
Title Effect of ethylene and culture environment on development of papaya nodal cultures
Journal name Plant Cell, Tissue and Organ Culture   Check publisher's open access policy
ISSN 0167-6857
Publication date 1997
Sub-type Article (original research)
DOI 10.1023/A:1005869211684
Volume 49
Issue 2
Start page 93
End page 100
Total pages 8
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Abstract Papaya (Carica papaya L.) nodal cultures modified the atmosphere of the headspace of the vessel used for culture maintenance by producing ethylene. Under culture maintenance nodal cultures grew poorly and leaves senesced. Incubating nodal cultures under a range of ethylene concentrations suggested that this poor performance was caused in part, by the production of ethylene and its accumulation in the headspace of the vessel. To further evaluate the role of ethylene accumulation in growth suppression, aminoethoxyvinylglycine (AVG), l-aminocyclopropane-l-carboxylic acid (ACC) and silver thiosulphate (STS), were added to the nutrient medium and ethylene measurements performed during culture growth. The ethylene-suppressant, AVG, (1.2 μM) and the ethylene-antagonist, STS, (0.3 mM) significantly improved nodal culture growth (283 and 289% respectively), leaf area production (350 and 211% respectively) and reduced leaf senescence, while the ethylene-precursor, ACC, (1.5 mM) significantly decreased culture growth (71%), leaf area production (88%) and promoted leaf senescence. Furthermore, nodal culture growth was significantly better at 20 °C than 30 °C since ethylene production and accumulation were less in these conditions. Better control or management of ethylene accumulation produces healthier nodal cultures for micro-propagation and may be a way of improving productivity of other papaya shoot culture systems.
Keyword Aminoethoxyvinylglycine
Head space gases
Silver thiosulphate
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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