RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var indica) somaclonal progenies

Godwin, I. D., Sangduen, N., Kunanuvatchaidach, R., Piperidis, G. and Adkins, S. W. (1997) RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var indica) somaclonal progenies. Plant Cell Reports, 16 5: 320-324. doi:10.1007/s002990050231


Author Godwin, I. D.
Sangduen, N.
Kunanuvatchaidach, R.
Piperidis, G.
Adkins, S. W.
Title RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var indica) somaclonal progenies
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 1997-02
Sub-type Article (original research)
DOI 10.1007/s002990050231
Volume 16
Issue 5
Start page 320
End page 324
Total pages 5
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar, indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphisms were found at 28 of the 45 bands among the somaclonal families, including both loss of parental bands, and the appearance of novel non-parental bands. Segregation data revealed both heterozygous and homozygous mutation events, with recessive mutations more prevalent than dominant. All somaclonal families differed significantly from the parental material, indicating that genomic alterations occurred in all families regardless of phenotype. None of the variant families could be regarded as isogenic lines of FR13A at the DNA level. However, some of the DNA level variation may be in highly repeated sequences with no phenotypic effects. The implications for somaclonal breeding and genetic engineering programs are discussed.
Keyword Oryza sativa
Rice
Somaclonal variation
Genetic integrity
RAPD analysis
DNA markers
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Thu, 10 Mar 2011, 11:01:56 EST