An efficient in vitro regeneration system for Australian-grown chickpea (Cicer arietinum) cultivars

Adkins, A. L., Godwin, I. D. and Adkins, S. W. (1995) An efficient in vitro regeneration system for Australian-grown chickpea (Cicer arietinum) cultivars. Australian Journal of Botany, 43 5: 491-497.


Author Adkins, A. L.
Godwin, I. D.
Adkins, S. W.
Title An efficient in vitro regeneration system for Australian-grown chickpea (Cicer arietinum) cultivars
Formatted title An efficient in vitro regeneration system for Australian-grown chickpea (Cicer arietinum) cultivars
Journal name Australian Journal of Botany
Publication date 1995
Sub-type Article
DOI 10.1071/BT9950491
Volume number 43
Issue number 5
ISSN 0067-1924; 1444-9862
Start page 491
End page 497
Total pages 7
Place of publication Collingwood, Vic., Australia
Publisher CSIRO Publishing
Language eng
Formatted abstract A comparison of methods for efficient in vitro regeneration of Australian-grown chickpea (Cicer arietinum L.) cultivars was undertaken. The most efficient regeneration system was one where immature cotyledon and embryonic axis explants, 14-21 days post-pollination, were cultivated on Murashige and Skoog's salts with Gamborg's vitamins, 1.0, 3.0 or 5.2 mg L-1 zeatin, 0 or 35 μg L-1 indole-3-acetic acid, 30 g L-1 sucrose and 8 g L-1 Phytagar. The first embryoid structures appeared after 2 weeks of culture at 25 ± 1°C in dim light (150 μmol m-2 s-1) and formed directly on the edges of the immature cotyledons or petiole stumps. Between 10 and 20 structures were produced on each cotyledon explant in two cultivars, however, the embryogenic structures which developed on cv. Narayen were more efficiently transformed into shoots than far cv. Amethyst. An efficient regeneration medium (2 mg L-1 naphthaleneacetic acid, 1/2 Murashige and Skoog's salts with Gamborg's vitamins, and 0.5 g L-1 activated charcoal) was used to develop a portion of the shoots into morphologically normal plants growing in a vermiculite and soil potting mix in a growth room. Less efficient in vitro regeneration was observed when hypocotyl and shoot sections, and shoot apices were induced to form callus and plants by organogenesis. These plants could not be established in a potting mix. The amount and type of callus produced varied between explant type and cultivar.
Keyword Somatic embryogenisis
Plant-regeneration
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collection: School of Agriculture and Food Sciences
 
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