Cyclic ADP-ribose regulation of ryanodine receptors involved in agonist evolved cytosolic Ca2+ oscillations in pancreatic acinar cells

Thorn, P., Gerasimenko, O. and Petersen, O. H. (1994) Cyclic ADP-ribose regulation of ryanodine receptors involved in agonist evolved cytosolic Ca2+ oscillations in pancreatic acinar cells. EMBO Journal, 13 9: 2038-2043.

Author Thorn, P.
Gerasimenko, O.
Petersen, O. H.
Title Cyclic ADP-ribose regulation of ryanodine receptors involved in agonist evolved cytosolic Ca2+ oscillations in pancreatic acinar cells
Journal name EMBO Journal   Check publisher's open access policy
ISSN 0261-4189
Publication date 1994-05
Sub-type Article (original research)
Volume 13
Issue 9
Start page 2038
End page 2043
Total pages 6
Place of publication London, United Kingdom
Publisher Nature
Language eng
Formatted abstract
We have investigated the role of the ryanodine-sensitive intracellular Ca2+ release channel (ryanodine receptor) in the cytosolic Ca2+ oscillations evoked in pancreatic acinar cells by acetylcholine (ACh) or cholecystokinin (CCK). Ryanodine abolished or markedly inhibited the agonist evoked Ca2+ spiking, but enhanced the frequency of spikes evoked by direct internal inositol trisphosphate (InsP3) application. We have also investigated the possibility that cyclic ADP-ribose (cADP-ribose), the putative second messenger controlling the ryanodine receptor, plays a role in Ca2+ oscillations. We found that cADP-ribose could itself induce repetitive Ca2+ spikes localized in the secretory pole and that these spikes were blocked by ryanodine, but also by the InsP3 receptor antagonist heparin. Our results indicate that both the ryanodine and the InsP3 receptors are involved in Ca2+ spike generation.
Keyword Acetylcholine
Ca2+ oscillations
Cholecystokinin
Cyclic ADP-ribose
Inositol trisphosphate
Ryanodine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Thu, 10 Mar 2011, 08:47:41 EST