Functional analysis of human bronchial mucosal T cells extracted with interleukin-2

Upham, J. W., McMenamin, C., Schon-Hegrad, M. A., Robinson, B. W. S. and Holt, P. G. (1994) Functional analysis of human bronchial mucosal T cells extracted with interleukin-2. American Journal of Respiratory and Critical Care Medicine, 149 6: 1608-1613.

Author Upham, J. W.
McMenamin, C.
Schon-Hegrad, M. A.
Robinson, B. W. S.
Holt, P. G.
Title Functional analysis of human bronchial mucosal T cells extracted with interleukin-2
Journal name American Journal of Respiratory and Critical Care Medicine   Check publisher's open access policy
ISSN 1073-449X
Publication date 1994-06
Sub-type Article (original research)
Volume 149
Issue 6
Start page 1608
End page 1613
Total pages 6
Place of publication New York, NY, United States
Publisher American Thoracic Society
Language eng
Abstract Functional analysis of T cells from the bronchial mucosa has been limited by difficulties in extracting T cells from this tissue. Because interleukin- 2 (IL-2) is chemotactic for T cells, we determined whether this cytokine could be used to extract T cells from human bronchial wall (BW). Fresh tissue was obtained from 21 patients undergoing surgery for malignancy. Within the BW, 95% of T cells stained for the memory/activation marker CD45RO. When BW sections were incubated with IL-2 for 24 h, 88 to 91% of T cells emigrated into the culture medium. Compared with autologous blood T cells (also exposed to IL-2), these BW T cells expressed CD2 at a greater intensity and showed a fourfold reduction in cloning efficiency in response to phytohemagglutinin, and T-cell clones derived from the BW population displayed a tendency for higher interferon-γ production. Furthermore, we were also able to extract and clone T cells from bronchoscopic biopsies in four subjects, suggesting that this method will provide a new avenue for examining T-cell function in airway inflammatory diseases.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 09 Mar 2011, 15:18:58 EST