Implementation of a large-scale ENU mutagenesis program: towards increasing the mouse mutant resource

Nolan, P. M., Peters, J., Vizor, L., Strivens, M., Washbourne, R., Hough, T., Wells, C., Glenister, P., Thornton, C., Martin, J., Fisher, E., Rogers, D., Hagan, J., Reavill, C., Gray, I., Wood, J., Spurr, N., Browne, M., Rastan, S., Hunter, J. and Brown, S. D. M. (2000) Implementation of a large-scale ENU mutagenesis program: towards increasing the mouse mutant resource. Mammalian Genome, 11 7: 500-506. doi:10.1007/s003350010096

Author Nolan, P. M.
Peters, J.
Vizor, L.
Strivens, M.
Washbourne, R.
Hough, T.
Wells, C.
Glenister, P.
Thornton, C.
Martin, J.
Fisher, E.
Rogers, D.
Hagan, J.
Reavill, C.
Gray, I.
Wood, J.
Spurr, N.
Browne, M.
Rastan, S.
Hunter, J.
Brown, S. D. M.
Title Implementation of a large-scale ENU mutagenesis program: towards increasing the mouse mutant resource
Journal name Mammalian Genome   Check publisher's open access policy
ISSN 0938-8990
Publication date 2000-07
Sub-type Article (original research)
DOI 10.1007/s003350010096
Volume 11
Issue 7
Start page 500
End page 506
Total pages 7
Place of publication Secaucus, NJ, United States
Publisher Springer
Language eng
Abstract Systematic approaches to mouse mutagenesis will be vital for future studies of gene function. We have begun a major ENU mutagenesis program incorporating a large genome-wide screen for dominant mutations. Progeny of ENU-mutagenized mice are screened for visible defects at birth and weaning, and at 5 weeks of age by using a systematic and semi-quantitative screening protocol - SHIRPA. Following this, mice are screened for abnormal locomotor activity and for deficits in prepulse inhibition of the acoustic startle response. Moreover, in the primary screen, blood is collected from mice and subjected to a comprehensive clinical biochemical analysis. Subsequently, secondary and tertiary screens of increasing complexity can be used on animals demonstrating deficits in the primary screen. Frozen sperm is archived from all the male mice passing through the screen. In addition, tail tips are stored for DNA. Overall, the program will provide an extensive new resource of mutant and phenotype data to the mouse and human genetics communities at large. The challenge now is to employ the expanding mouse mutant resource to improve the mutant map of the mouse. An improved mutant map of the mouse will be an important asset in exploiting the growing gene map of the mouse and assisting with the identification of genes underlying novel mutations - with consequent benefits for the analysis of gene function and the identification of novel pathways.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Wed, 09 Mar 2011, 14:34:19 EST