A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse

Nolan, P. M., Peters, J., Strivens, M., Rogers, D., Hagan, J., Spurr, N., Gray, I. C., Vizor, L., Brooker, D., Whitehill, E., Washbourne, R., Hough, T., Greenaway, S., Hewitt, M., Liu, X. H., McCormack, S., Pickford, K., Selley, R., Wells, C., Tymowska-Lalanne, Z., Roby, P., Glenister, P., Thornton, C., Thaung, C., Stevenson, J. A., Arkell, R., Mburu, P., Hardisty, R., Kiernan, A., Erven, H., Steel, K. P., Voegeling, S., Guenet, J. L., Nickols, C., Sadri, R., Naase, M., Isaacs, A., Davies, K., Browne, M., Fisher, E. M. C., Martin, J., Rastan, S., Brown, S. D. M. and Hunter, J. (2000) A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse. Nature Genetics, 25 4: 440-443. doi:10.1038/78140

Author Nolan, P. M.
Peters, J.
Strivens, M.
Rogers, D.
Hagan, J.
Spurr, N.
Gray, I. C.
Vizor, L.
Brooker, D.
Whitehill, E.
Washbourne, R.
Hough, T.
Greenaway, S.
Hewitt, M.
Liu, X. H.
McCormack, S.
Pickford, K.
Selley, R.
Wells, C.
Tymowska-Lalanne, Z.
Roby, P.
Glenister, P.
Thornton, C.
Thaung, C.
Stevenson, J. A.
Arkell, R.
Mburu, P.
Hardisty, R.
Kiernan, A.
Erven, H.
Steel, K. P.
Voegeling, S.
Guenet, J. L.
Nickols, C.
Sadri, R.
Naase, M.
Isaacs, A.
Davies, K.
Browne, M.
Fisher, E. M. C.
Martin, J.
Rastan, S.
Brown, S. D. M.
Hunter, J.
Title A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse
Journal name Nature Genetics   Check publisher's open access policy
ISSN 1061-4036
Publication date 2000-08
Sub-type Article (original research)
DOI 10.1038/78140
Volume 25
Issue 4
Start page 440
End page 443
Total pages 4
Place of publication New York, NY, United States
Publisher Nature
Language eng
Abstract As the human genome project approaches completion, the challenge for mammalian geneticists is to develop approaches for the systematic determination of mammalian gene function. Mouse mutagenesis will be a key element of studies of gene function. Phenotype-driven approaches using the chemical mutagen ethylnitrosourea (ENU) represent a potentially efficient route for the generation of large numbers of mutant mice that can be screened for novel phenotypes. The advantage of this approach is that, in assessing gene function, no a priori assumptions are made about the genes involved in any pathway. Phenotype-driven mutagenesis is thus an effective method for the identification of novel genes and pathways. We have undertaken a genome-wide, phenotype-driven screen for dominant mutations in the mouse. We generated and screened over 26,000 mice, and recovered some 500 new mouse mutants. Our work, along with the programme reported in the accompanying paper, has led to a substantial increase in the mouse mutant resource and represents a first step towards systematic studies of gene function in mammalian genetics.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Wed, 09 Mar 2011, 14:34:13 EST