The relationship between allergen-induced tissue eosinophilia and markers of repair and remodeling in human atopic skin

Phipps, S., Ying, S., Wangoo, A., Ong, Y. E., Levi-Schaffer, F. and Kay, A. B. (2002) The relationship between allergen-induced tissue eosinophilia and markers of repair and remodeling in human atopic skin. Journal of Immunology, 169 8: 4604-4612.

Author Phipps, S.
Ying, S.
Wangoo, A.
Ong, Y. E.
Levi-Schaffer, F.
Kay, A. B.
Title The relationship between allergen-induced tissue eosinophilia and markers of repair and remodeling in human atopic skin
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2002-10
Sub-type Article (original research)
Volume 169
Issue 8
Start page 4604
End page 4612
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Several in vitro studies suggest that eosinophils may play a role in fibrosis, remodeling, and repair processes associated with IgE-mediated hypersensitivity. However, the relationship in vivo, between allergen-induced tissue eosinophilia and markers of repair has yet to be established in human atopic subjects. Using the allergen-induced cutaneous late-phase reaction as a model of allergic inflammation, we have tested the hypothesis that eosinophil-derived TGF-β1 and IL-13 are temporarily associated with myofibroblast formation and deposition of tenascin and procollagen 1. Biopsies were taken from atopic volunteers at 1, 3, 6, 24, 48, and 72 h after intradermal allergen challenge and were examined by immunohistochemistry. Following the peak of the late-phase reaction (6 h) there were persisting TGF-β1 eosinophils, α-smooth muscle actin+ myofibroblasts, tenascin immunoreactivity, and procollagen-I+ cells 24-48 h postchallenge. Direct evidence of generation of repair markers was obtained by coculture of eosinophils and fibroblasts. This resulted in α-smooth muscle actin immunoreactivity that was inhibitable by neutralizing Abs to TGF-β as well as production of tenascin transcripts and protein product. TGF-β1 and IL-13 also induced tenascin expression. We conclude that TGF-β1 and IL-13, provided partially by eosinophils, contribute to repair and remodeling events in allergic inflammation in human atopic skin.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 09 Mar 2011, 14:31:49 EST