Early production of thymic stromal lymphopoietin precedes infiltration of dendritic cells expressing its receptor in allergen-induced late phase cutaneous responses in atopic subjects

Corrigan, C. J., Jayaratnam, A., Wang, Y., Liu, Y., Malefyt, R. D., Meng, Q., Kay, A. B., Phipps, S., Lee, T. H. and Ying, S. (2009) Early production of thymic stromal lymphopoietin precedes infiltration of dendritic cells expressing its receptor in allergen-induced late phase cutaneous responses in atopic subjects. Allergy, 64 7: 1014-1022. doi:10.1111/j.1398-9995.2009.01947.x


Author Corrigan, C. J.
Jayaratnam, A.
Wang, Y.
Liu, Y.
Malefyt, R. D.
Meng, Q.
Kay, A. B.
Phipps, S.
Lee, T. H.
Ying, S.
Title Early production of thymic stromal lymphopoietin precedes infiltration of dendritic cells expressing its receptor in allergen-induced late phase cutaneous responses in atopic subjects
Journal name Allergy   Check publisher's open access policy
ISSN 0105-4538
1398-9995
Publication date 2009-07
Sub-type Article (original research)
DOI 10.1111/j.1398-9995.2009.01947.x
Volume 64
Issue 7
Start page 1014
End page 1022
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Background:  Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-7-like cytokine that triggers dendritic cell-mediated T helper (Th)2 inflammatory responses through a receptor consisting of a heterodimer of the IL-7 receptor alpha (IL-7Rα) chain and the TSLP receptor (TSLPR), which resembles the cytokine receptor common gamma chain. Dendritic cells activated by TSLP prime development of CD4+ T cells into Th2 cells contributing to the pathogenesis of allergic inflammation. We hypothesized that allergen exposure induces expression of TSLP and results in recruitment of TSLPR bearing cells in the cutaneous allergen-induced late-phase reaction (LPR) in atopic subjects.
Methods:  Skin biopsies were obtained from atopic subjects (n = 9) at various times after cutaneous allergen challenge. In situ hybridization and immunohistochemistry were used to determine TSLP mRNA expression and to measure infiltration of TSLPR+ DC in skin LPR. RT-PCR and flow cytometry were employed to analyse TSLPR expression on isolated blood DC.
Results:  Allergen-induced skin TSLP expression occurred as early as 1 h after allergen challenge, whereas TSLPR+ and CD11c+ cells infiltrated relatively late (24–48 h). The majority of TSLPR+ cells were DC co-expressing blood DC antigen-1 (BDCA-1) or BDCA-2. Freshly isolated blood DC expressed both TSLPR and IL-7Rα chains. Maturation and stimulation with TSLP or polyriboinosinic–polyribocytidylic acid in vitro upregulated the expression of both TSLPR and IL-7Rα chains in DC but not in chemoattractant receptor-homologous molecule expressed on Th2 cells+ CD4+ T cells.
Conclusion:  The data suggest that TSLP plays a role in augmenting, through DC recruitment and activation, the development of Th2-type T cells in allergic inflammation.
Keyword Allergic inflammation
Dendritic cells
Thymic stromal lymphopoietin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 09 Mar 2011, 14:28:30 EST