Synthesis of siRNA Polyplexes Adopting a Combination of RAFT Polymerization and Thiol-ene Chemistry

Valade, David, Boyer, Cyrille, Davis, Thomas P. and Bulmus, Volga (2009) Synthesis of siRNA Polyplexes Adopting a Combination of RAFT Polymerization and Thiol-ene Chemistry. Australian Journal of Chemistry, 62 10: 1344-1350. doi:10.1071/CH09208


Author Valade, David
Boyer, Cyrille
Davis, Thomas P.
Bulmus, Volga
Title Synthesis of siRNA Polyplexes Adopting a Combination of RAFT Polymerization and Thiol-ene Chemistry
Journal name Australian Journal of Chemistry   Check publisher's open access policy
ISSN 0004-9425
1445-0038
Publication date 2009
Sub-type Article (original research)
DOI 10.1071/CH09208
Volume 62
Issue 10
Start page 1344
End page 1350
Total pages 7
Place of publication Collingwood, VIC, Australia
Publisher C S I R O Publishing
Language eng
Abstract Block copolymers of allyl methacrylate and N-(2-hydroxypropyl) methacrylamide (HPMA) with different block lengths have been synthesized by reversible additionfragmentation chain transfer polymerization. Allyl groups were modified with cysteamine, via a thiol-ene photoreaction, with a high efficiency (∼100%) as evidenced by NMR spectroscopy, yielding cationic copolymers of HPMA. Polyelectrolyte complexes of small interfering RNAs (siRNA) and the cationic block copolymers were then formed at an N/P ratio between 1 and 4 depending on the block length of the copolymers. Increasing the length of the hydrophilic block was found to decrease the efficiency of siRNA complexation. The hydrodynamic diameter of the polyplexes in 130 mM buffer solution was less than 100 nm.
Keyword CROSS-LINKED MICELLES
BLOCK-COPOLYMERS
RADICAL POLYMERIZATION
ALLYL METHACRYLATE
DELIVERY-SYSTEMS
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 09 Mar 2011, 13:49:29 EST