Isolation and characterization of acidophilic bacterium from Gaofeng Mine in China

Yang, Yu, Qian, Lin, Shi, Wu Yang, Peng, Hong and Qiu, Guan-zhou (2008) Isolation and characterization of acidophilic bacterium from Gaofeng Mine in China. Transactions of Nonferrous Metals Society of China, 18 5: 1253-1257. doi:10.1016/S1003-6326(08)60212-5

Author Yang, Yu
Qian, Lin
Shi, Wu Yang
Peng, Hong
Qiu, Guan-zhou
Title Isolation and characterization of acidophilic bacterium from Gaofeng Mine in China
Journal name Transactions of Nonferrous Metals Society of China   Check publisher's open access policy
ISSN 1003-6326
Publication date 2008-10
Sub-type Article (original research)
DOI 10.1016/S1003-6326(08)60212-5
Volume 18
Issue 5
Start page 1253
End page 1257
Total pages 5
Place of publication Changsha, China
Publisher Nonferrous Metals Society of China
Language eng
Abstract An acidophilic, chemolithotrophic and ferrous oxidizing bacterium strain GF was isolated from the acid mine drainage (AMD) of Gaofeng Mine, Guangxi Province, China using 9K enrichment medium, and then purified on solid ferrous-agarose medium. The physiological experiments show that it can use ferrous or sulfur as sole energy and a low level (0.1%, w/v) of peptone can accelerate the growth of the isolated strain. The optimum pH and temperature for growth are 2.0 and 30 °C, respectively. The isolated strain shares 99.64% identities of 16S rRNA gene with the type strain Acidthiobacillus ferrooxidans ATCC 23270 and 100% identities of iro gene (CDS) with A. ferrooxidans strain Fe-1. These results show that the strain can be considered as Acidthiobacillus ferrooxidans. Because of the high activity of oxidizing ferrous and sulfide mineral, strain GF was used in bioleaching of marmatite. The Zn concentration is 0.273 g/L under the steriled control and 7.30 g/L with adapted GF strain incubated after 29 d in leaching marmatite. The isolated strain GF can be used to leach marmatite in industry application.
Keyword acid mine drainage
Acidthiobacillus ferrooxidans
iron-oxidizing bacteria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Created: Wed, 09 Mar 2011, 13:08:57 EST