Characterization, inheritance, and molecular study of opaque leaf mutant in mungbean (Vigna radiata (L.) Wilczek)

Rungnoi, Ornuma, Chanprem, Sonthichai, Toojinda, Theerayut, Godwin, Ian, Lambrides, Chris and Srinives, Peerasak (2010) Characterization, inheritance, and molecular study of opaque leaf mutant in mungbean (Vigna radiata (L.) Wilczek). Journal of Crop Science and Biotechnology, 13 4: 219-226. doi:10.1007/s12892-010-0059-y


Author Rungnoi, Ornuma
Chanprem, Sonthichai
Toojinda, Theerayut
Godwin, Ian
Lambrides, Chris
Srinives, Peerasak
Title Characterization, inheritance, and molecular study of opaque leaf mutant in mungbean (Vigna radiata (L.) Wilczek)
Formatted title
Characterization, inheritance, and molecular study of opaque leaf mutant in mungbean (Vigna radiata (L.) Wilczek)
Journal name Journal of Crop Science and Biotechnology   Check publisher's open access policy
ISSN 1975-9479
2005-8276
Publication date 2010-12
Sub-type Article (original research)
DOI 10.1007/s12892-010-0059-y
Volume 13
Issue 4
Start page 219
End page 226
Total pages 8
Place of publication Suwon, Korea
Publisher Han'gug Jagmul Haghoe
Collection year 2011
Language eng
Formatted abstract
Chlorophyll-deficiency mutants are useful as genetic markers and as materials for studying the photosynthesis process. We characterized the inheritance of the gene controlling an opaque leaf (OL) trait in mungbean (Vigna radiata (L.) Wilczek). An F2 population was developed from the cross between the OL mutant and ‘Berken’, an Australian mungbean cultivar, to study inheritance and molecular tagging. The population was advanced by selfing to produce F5 lines from which two normal lines and two OL lines were randomly chosen to study chlorophyll content, seed growth and development, and seed cell morphology. The chlorophyll content in opaque leaf was lower than normal and thus was expected to have lower photosynthetic activity. This resulted in yellowish and shrinking pods and seeds within 15 days after flowering, while those from normal plants extended their growth up to 18 days. The cotyledon transfer cells of the OL plants deformed at 12 days and deteriorated at 14 days after flowering. The OL trait was controlled by a single recessive ol gene which was independent from the genes controlling petiole color and growth habit. We used 193 AFLP primer combinations to tag this gene and found that the marker AGG/ATA was linked with the ol gene at a distance of 3.4 cM.
© Springer
Keyword Characterization
Inheritance
Molecular marker
References – – – – – Vigna radiata Opaque leaf
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Wed, 09 Mar 2011, 12:57:13 EST by Marie-Louise Moore on behalf of School of Land, Crop and Food Sciences