Analysis of apoptosis morphology in Epstein-Barr virus positive and negative Burkitt's lymphoma cells

Ishii, H., Gobe, G. C., Joshita, T., Kurabayashi, Y., Hosomura, Y. and Kameya, T. (1997) Analysis of apoptosis morphology in Epstein-Barr virus positive and negative Burkitt's lymphoma cells. Archives of Histology and Cytology, 60 2: 143-152. doi:10.1679/aohc.60.143


Author Ishii, H.
Gobe, G. C.
Joshita, T.
Kurabayashi, Y.
Hosomura, Y.
Kameya, T.
Title Analysis of apoptosis morphology in Epstein-Barr virus positive and negative Burkitt's lymphoma cells
Journal name Archives of Histology and Cytology   Check publisher's open access policy
ISSN 0914-9465
Publication date 1997-06
Sub-type Article (original research)
DOI 10.1679/aohc.60.143
Volume 60
Issue 2
Start page 143
End page 152
Total pages 10
Place of publication Niigata, Japan
Publisher International Society of Histology and Cytology
Language eng
Formatted abstract
In recent studies of cycloheximide (CHX)induced apoptosis in sublines of established Burkitt's lymphoma cell lines (BJA-B) both with and without Epstein-Barr virus (EBV) infection, we noticed two distinct types of apoptosis morphology. In the present paper, we have classified these, and further carried out a statistical analysis of their incidence in untreated and CHX-treated EBV-free (EBV(-)) and EBV-infected (EBV(+)) BJA-B cells. Classification: Both types of apoptosis morphology demonstrated typical nuclear and cytoplasmic condensation. However, 'Type 1 apoptotic cells' (AP1) maintained a spherical or ovoid shape, but 'Type 2 apoptotic cells' (AP2) were typified by the lobulation of their nuclear and cytoplasmic structures to form 'clover leaf' shapes. Statistical analysis of incidence: The numbers of AP1 and AP2 cells were analysed using a χ2 test, with results as follows: EBV(-) cells underwent AP1 in preference to AP2 (90.5% versus 9.5%) (p<:0.001), whilst EBV(+) cells had comparably more AP2, making AP1 and AP2 approximately equal (49.3% versus 50.7%) (p>0.l). In EBV(-) cells, treatment with CHX had little effect on the ratios of differing apoptotic morphology. In contrast, in the EBV(+) cells, cell death was altered from AP2 (50.7%→ 25.2%) towards AP1(49.3%→74.8%) (p<0.001). We propose that cellular proteins known to be associated with EBV infection not only protect the cells from apoptosis, but also affect the phenotype of apoptosis. This knowledge may be useful for defining possible mechanisms of apoptosis induction and/or inhibition in specific models.
Keyword Apoptosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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