Cell death induced in a murine mastocytoma by 42-47°C heating in vitro: Evidence that the form of death changes from apoptosis to necrosis above a critical heat load

Harmon, B. V., Corder, A. M., Collins, R. J., Gobe, G. C., Allen, J., Allan, D. J. and Kerr, J. F. R. (1990) Cell death induced in a murine mastocytoma by 42-47°C heating in vitro: Evidence that the form of death changes from apoptosis to necrosis above a critical heat load. International Journal of Radiation Biology, 58 5: 845-858. doi:10.1080/09553009014552221


Author Harmon, B. V.
Corder, A. M.
Collins, R. J.
Gobe, G. C.
Allen, J.
Allan, D. J.
Kerr, J. F. R.
Title Cell death induced in a murine mastocytoma by 42-47°C heating in vitro: Evidence that the form of death changes from apoptosis to necrosis above a critical heat load
Journal name International Journal of Radiation Biology   Check publisher's open access policy
ISSN 0955-3002
Publication date 1990-11
Sub-type Article (original research)
DOI 10.1080/09553009014552221
Volume 58
Issue 5
Start page 845
End page 858
Total pages 14
Place of publication London, United Kingdom
Publisher Informa Healthcare
Language eng
Abstract The pathogenesis of heat-induced cell death is controversial. Categorizing the death occurring after various heat loads as either apoptosis or necrosis might help to elucidate this problem, since it has been shown that these two processes differ in their mode of initiation as well as in their morphological and biochemical features. Log-phase cultures of mastocytoma P-815 x 2.1 were heated at temperatures ranging from 42 to 47°C for 30 min. After 42°C heating a slight increase in apoptosis was observed morphologically. However, after heating at 43, 43.5 and 44°C, there was marked enhancement of apoptosis, and electrophoresis of DNA showed characteristic internucleosomal cleavage. With heating at 45°C both apoptosis and necrosis were enhanced, whereas at 46 and 47°C only necrosis was produced. DNA extracted from the 46 and 47°C cultures showed virtually no degradation, which contrasts with the random DNA breakdown observed in necrosis produced by other types of injury; lysosomal enzymes released during heat-induced necrosis may be inactivated at the higher temperatures. It is suggested that apoptosis following heating may be triggered either by a limited increase in cytosolic calcium levels resulting from mild membrane changes or by DNA damage. Necrosis, on the other hand, is likely to be a consequence of severe membrane disruption.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 09 Mar 2011, 08:33:10 EST