Expression and localization of the retinoblastoma gene during radiation-induced apoptosis in neonatal rat kidney

Gobe, G. C., Halliday, J., Dunn, I. S., Harmon, B. V., Schoch, E., Khan, S. M. and Allan, D. J. (1997) Expression and localization of the retinoblastoma gene during radiation-induced apoptosis in neonatal rat kidney. Experimental Cell Research, 235 2: 354-361. doi:10.1006/excr.1997.3682


Author Gobe, G. C.
Halliday, J.
Dunn, I. S.
Harmon, B. V.
Schoch, E.
Khan, S. M.
Allan, D. J.
Title Expression and localization of the retinoblastoma gene during radiation-induced apoptosis in neonatal rat kidney
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
1090-2422
Publication date 1997-09-01
Sub-type Article (original research)
DOI 10.1006/excr.1997.3682
Volume 235
Issue 2
Start page 354
End page 361
Total pages 8
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
An in vivo neonatal rat kidney model was used to study an association between expression and localization of the retinoblastoma tumor-suppressor gene (Rb), or its protein product (pRb), and localization of radiation- induced apoptosis. The rat kidney has two distinct zones of differentiation at birth - an outer nephrogenic zone, in which cells are undifferentiated and new nephrons are forming, and a differentiated zone internal to this zone that has essentially the adult kidney form. At 6 h after radiation (5 Gy), high levels of relatively synchronous apoptosis are induced in the nephrogenic zone, with little effect on the differentiated zone, and proliferation in the nephrogenic zone is almost totally inhibited by radiation treatment, again with little effect in the differentiated area. We have used our knowledge of this model to analyze control (sham-treated) and irradiated renal tissue for Rb mRNA transcript levels and localization (Northern blot and in situ hybridization (ISH)), pRb expression (Western blot and immunolocalization), apoptosis and mitosis (light and electron microscopy, and DNA gel electrophoresis for apoptosis), and cells in S-phase ([ 3H]thymidine uptake and autoradiography). Northern blots showed no detectable alteration in Rb transcript levels between control and irradiated tissues, whereas Western blots indicated increased expression of pRb in protein extracted from irradiated kidney compared with controls. ISH confirmed that Rb transcripts were not substantially altered in the nephrogenic and differentiated zones in control versus irradiated renal tissue. Immunolocalization of pRb demonstrated little effect in the differentiated zone, but in the nephrogenic zone pRb expression was increased, especially the S-shaped prenephrons, and was also found in many, but not all, apoptotic cells in this zone. The results link radiation- induced apoptosis and increased pRb expression in a zone of the neonatal kidney having a low level of cell differentiation.
Keyword Retinoblastoma tumor-suppressor gene
pRb
Radiation- induced apoptosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 09 Mar 2011, 18:32:38 EST