Effects of hypoxia on morphological and biochemical characteristics of renal epithelial cell and tubule cultures

Allen, J., Winterford, C., Axelsen, R. A. and Gobe, G. C. (1992) Effects of hypoxia on morphological and biochemical characteristics of renal epithelial cell and tubule cultures. Renal Failure, 14 4: 453-460. doi:10.3109/08860229209047652


Author Allen, J.
Winterford, C.
Axelsen, R. A.
Gobe, G. C.
Title Effects of hypoxia on morphological and biochemical characteristics of renal epithelial cell and tubule cultures
Journal name Renal Failure   Check publisher's open access policy
ISSN 0886-022X
Publication date 1992
Sub-type Article (original research)
DOI 10.3109/08860229209047652
Volume 14
Issue 4
Start page 453
End page 460
Total pages 8
Place of publication New York, NY, United States
Publisher Informa Healthcare
Language eng
Abstract Apoptotic cell death plays an important role in the pathogenesis of renal atrophy in diseases of the kidney involving chronic mild ischemia. The present study constitutes an in vitro model of these diseases and assesses the modes of cell death involved after hypoxic treatment of renal epithelium. Cultures of MDCK cells or primary cultures of rat renal parenchymal tubules were treated in either a physiological or a hypoxic atmosphere. Cultures were collected before treatment and at 24 h and 48 h, for morphological and biochemical studies. Both apoptosis and necrosis were observed at significantly increased levels by 48 h of hypoxia in the MDCK cell cultures. DNA gel electrophoresis patterns supported these findings. Experiments using tubule cultures demonstrated that, during the 48 h of study, tubular epithelial cells in the center of the control tubule structures died by apoptosis, possibly as a result of mild oxygen and/or nutrient depletion. With added hypoxic treatment, however, the entire tubule structure became necrotic. Results are similar to those found during in vivo studies, thus providing in vitro models that may be developed further to define factors in the pathogenesis of some renal diseases.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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