Zinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators

Gauci, Victoria J., Beckhouse, Anthony G., Lyons, Victoria, Beh, Eric J., Rogers, Peter J., Dawes, Ian W. and Higgins, Vincent J. (2009) Zinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators. Fems Yeast Research, 9 8: 1187-1195.


Author Gauci, Victoria J.
Beckhouse, Anthony G.
Lyons, Victoria
Beh, Eric J.
Rogers, Peter J.
Dawes, Ian W.
Higgins, Vincent J.
Title Zinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators
Formatted title Zinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators
Journal name Fems Yeast Research   Check publisher's open access policy
ISSN 1567-1356
1567-1364
Publication date 2009-12
Sub-type Article (original research)
DOI 10.1111/j.1567-1364.2009.00557.x
Volume 9
Issue 8
Start page 1187
End page 1195
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract During the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a 'sluggish' or a 'stuck' ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis identified the stress-response element (STRE) motif, recognized by Msn2/4p, and was significantly enriched in the promoters of genes induced by zinc deficiency. An investigation using genome-wide analyses revealed a distinct regulon consisting of STRE-containing genes whose zinc-responsive expression was abolished in an msn2 msn4 double mutant. An STRE-driven lacZ reporter construct confirmed that expression of the genes within this regulon was perturbed by the deletion of MSN2 and MSN4 and also implicated Hog1p as a contributing factor. This research provides a better understanding of the molecular mechanisms involved in the yeast response to zinc deficiency during fermentation.
Keyword Zinc
Msn2/4p
Zap1p
Saccharomyces cerevisiae
Fermentation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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