A gene from Alcaligenes denitrificans that confers albicidin resistance by reversible antibiotic binding

Basnayake, W. V. S. and Birch, Robert G. (1995) A gene from Alcaligenes denitrificans that confers albicidin resistance by reversible antibiotic binding. Microbiology, 141 3: 551-560.

Author Basnayake, W. V. S.
Birch, Robert G.
Title A gene from Alcaligenes denitrificans that confers albicidin resistance by reversible antibiotic binding
Formatted title


Journal name Microbiology   Check publisher's open access policy
ISSN 1350-0872
1465-2080
Publication date 1995-01-01
Sub-type Article (original research)
Volume 141
Issue 3
Start page 551
End page 560
Total pages 10
Place of publication Reading, Berks United Kingdom
Publisher Society for General Microbiology
Collection year 1995
Language eng
Subject 270300 Microbiology
Formatted abstract
Albicidin antibiotics specifically block prokaryote DNA replication. The albicidin resistance gene (albB) cloned from a soil isolate of Alcaligenes denitrificans encodes a 23 kDa protein capable of detoxifying albicidin by reversible binding. This mechanism operates intracellularly to protect DNA replication in albicidin-sensitive Escherichia coli expressing the cloned resistance gene, which can be induced fivefold in the presence of 1.5 μg albicidin ml-1 in the surrounding medium. The coding region of 621 bp has regions with partial DNA sequence homology to an albicidin resistance gene (albA) from Klebsiella oxytoca, but with rearrangements and frame-shifts resulting in loss of protein homology. There is a short region of N-terminal homology between the albicidin resistance (Alb(r)) proteins from A. denitrificans and K. oxytoca, although the two genes use different codons for shared amino acids. The N-terminal homology suggested a common functional domain; this was confirmed by deletion analysis, translational fusions and albicidin binding by a synthetic oligopeptide. Antibiotic binding provides a high level of albicidin resistance in E. coli. The gene appears to be a useful candidate for transfer to plants to protect plastid DNA replication from inhibition by albicidin phytotoxins involved in sugarcane leaf scald disease.
Keyword Alb gene
Albicidin Resistance
Alcaligenes denitrificanes
Antibiotic binding
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 08 Mar 2011, 21:07:10 EST by Shiromani Basnayake on behalf of Botany, Department of