An efficient transformation system for the Australian rice cultivar, Jarrah

Abedinia, M., Henry, R. J., Blakeney, A. B. and Lewin, L. (1997) An efficient transformation system for the Australian rice cultivar, Jarrah. Australian Journal of Plant Physiology, 24 2: 133-141.


Author Abedinia, M.
Henry, R. J.
Blakeney, A. B.
Lewin, L.
Title An efficient transformation system for the Australian rice cultivar, Jarrah
Journal name Australian Journal of Plant Physiology   Check publisher's open access policy
Publication date 1997
Sub-type Article
DOI 10.1071/PP96071
Volume number 24
Issue number 2
ISSN 0310-7841
Start page 133
End page 141
Total pages 9
Place of publication Collingwood, Vic., Australia
Publisher CSIRO Publishing
Language eng
Formatted abstract A rapid and efficient transformation system for the generation of large numbers of transformed, fertile, transgenic rice (Oryza sativa L.) plants of the Australian rice cultivar, Jarrah, is described. Embryogenic callus pieces derived from mature seeds were bombarded with gold particles coated with DNA. Two plasmids were used, one containing a gene encoding hygromycin phosphotransferase (hph, conferring hygromycin resistance) as a selectable marker and the other containing uidA (gus) as a reporter gene. The calli were selected for their resistance to hygromycin. DNA uptake, integration and expression of the hph and gus gene in selected rice were investigated by various PCR methods and dot blot and Southern analysis of genomic DNA extracted from transformed rice plants. On average one independently transformed hygromycin resistant plant was recovered from every 2.4 pieces of callus bombarded. Selection with Biolaphos using the Bar gene as selectable marker was not successful in this system.
Keyword Transgenic rice
Oryza sativa
Particle bombardment
Hygromycin resistance
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Journal title since 2002: Functional Plant Biology (1445-4408; 1445-4416)

Document type: Journal Article
Sub-type: Article
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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