Validating feathers for the field diagnosis of highly pathogenic avian influenza subtype H5N1

Nuradji, Harimurti, Wibawa, Hendra, Lowther, Sue, Payne, Jean, Rookes, Jennifer, Bingham, John and Meers, Joanne (2010). Validating feathers for the field diagnosis of highly pathogenic avian influenza subtype H5N1. In: Global Biosecurity 2010, Brisbane, QLD, Australia, (149-149). 28 February-3 March 2010.

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
GB2010_Final_Handbook.pdf HERDC peer review evidence and Full text - not publicly available application/pdf 8.82MB 0
Author Nuradji, Harimurti
Wibawa, Hendra
Lowther, Sue
Payne, Jean
Rookes, Jennifer
Bingham, John
Meers, Joanne
Title of paper Validating feathers for the field diagnosis of highly pathogenic avian influenza subtype H5N1
Conference name Global Biosecurity 2010
Conference location Brisbane, QLD, Australia
Conference dates 28 February-3 March 2010
Publication Year 2010
Sub-type Poster
Start page 149
End page 149
Total pages 1
Language eng
Formatted Abstract/Summary
Highly pathogenic avian influenza (HPAI) virus subtype H5N1 has resulted in serious consequences on public health, economics and animal health around the world. Active and passive surveillance based on rapid and accurate diagnosis plays a critical role in controlling this disease. Currently, cloacal and oropharyngeal swabs are the most commonly used sample types in rapid antigen detection tests. However, this approach may not provide reliable results because of bacterial contamination and the low levels of virus present in swabs. Several studies have reported high concentrations of H5N1 antigen in feather tissues. The aim of our study is to determine the distribution in chicken and duck feathers of an Indonesian duck-derived H5N1 virus and to validate feathers as a sample material for the field diagnosis of HPAI. Groups of 14 chickens and 14 ducks were challenged with a subclade 2.1.1 H5N1 virus. Cloacal swabs, oropharyngeal swabs and feathers were collected prior to challenge and at daily intervals from days 1 to 18 post-challenge. The concentration of virus in each sample type will be assessed by virus isolation and titration. Skin samples were collected for assessment of antigen by immunohistochemistry. The suitability of feathers for use as samples in rapid antigen detection tests will be assessed. This work has the potential to make a significant contribution to future surveillance and control of HPAI.
Subjects 0601 Biochemistry and Cell Biology
Q-Index Code EX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Presented as Poster no. 21.

Document type: Conference Paper
Collections: Non HERDC
School of Veterinary Science Publications
Version Filter Type
Citation counts: Google Scholar Search Google Scholar
Created: Mon, 07 Mar 2011, 15:51:55 EST by Assoc Prof Joanne Meers on behalf of School of Veterinary Science